| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 83,579 | (A)7→6 | intergenic (‑50/+43) | leuA ← / ← leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 83,573 | 0 | A | . | 94.4% | 61.6 / ‑1.3 | 18 | intergenic (‑44/+49) | leuA/leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (7/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGA > NC_000913/83468‑83682 | gCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGtttt > 2:472654/1‑133 (MQ=255)gCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGtttt > 1:466650/1‑133 (MQ=255) ccTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACt > 2:113060/1‑133 (MQ=255) cTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg > 1:474803/1‑133 (MQ=255) cACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGc < 2:466650/133‑1 (MQ=255) cGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTTGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATAGCGCGGGTTTTTTGTTTGACTGCGTGCTgg > 1:376387/1‑133 (MQ=255) tCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt < 1:472654/133‑1 (MQ=255) tCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt < 1:303873/133‑1 (MQ=255) gcgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa > 2:37122/1‑133 (MQ=255) gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATg < 1:470156/133‑1 (MQ=255) gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATg < 1:86522/133‑1 (MQ=255) gAAAATAATGACTTGCTGGCTCATGGTTTTGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATAGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt < 2:376387/133‑1 (MQ=255) gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt < 1:113060/133‑1 (MQ=255) gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt < 1:293578/133‑1 (MQ=255) aatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg > 2:460909/1‑133 (MQ=255) gTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGAt < 1:12954/133‑1 (MQ=255) gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCAT‑AAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGt < 1:37122/133‑1 (MQ=255) tttAGAGCTCCTCGCCTCAGGCTTAAAAAAA‑CCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGa < 1:219399/133‑1 (MQ=255) | GCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGA > NC_000913/83468‑83682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |