Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,367	Δ1 bp	coding (2586/2868 nt)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,364	0	C	.	100.0%	198.9 / NA	47	coding (2589/2868 nt)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/33);  total (14/33)

GTACTTCTCCCGGGAGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGA  >  NC_000913/1253232‑1253492                                                                                                                                     |                                                                                                                                 gTACTTCTCCTGGGAATTGTTCAACAGACATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACGc                                                                                                                                  <  2:186272/133‑1 (MQ=255)  tACTTCTCCCGGGAGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACGcc                                                                                                                                 >  1:47787/1‑133 (MQ=255)  tACTTCTCCCGGGAGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTAGCCTGCGGAGATTTCACACCCCAGTCTCATGCTGGCACGcc                                                                                                                                 >  2:159518/1‑133 (MQ=255)            gggAGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTTGCTGTTGCCTGCGGTCATGTCATAGCCAAGTAGCATGCTGGAACG‑CTCAGCAGCGAt                                                                                                                      >  2:219551/1‑133 (MQ=255)            gggAGTTGATCAACACATCTTCTGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGAt                                                                                                                      <  1:244671/133‑1 (MQ=255)             ggAGTTGTTCTACAGCTATTCGGTTTCCACTGTAAACTCGCAGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGAtt                                                                                                                     <  2:140529/133‑1 (MQ=255)               aGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCg                                                                                                                   >  1:433930/1‑133 (MQ=255)                 ttgttCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGTTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTa                                                                                                                 >  2:514758/1‑133 (MQ=255)                  tgttCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAg                                                                                                                <  1:382331/133‑1 (MQ=255)                       aaCAGATATTCGGTATCCCCTGAACACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATCCGTAGTGAtt                                                                                                           <  2:237392/133‑1 (MQ=255)                       aaCAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGAtt                                                                                                           <  2:273927/133‑1 (MQ=255)                       aaCAGATATATGGTAACCCCTGAAAAATAGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGAtt                                                                                                           <  2:237070/133‑1 (MQ=255)                         cAGATATTTGGTATCGCCTGAATACTCGCGAATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCa                                                                                                         <  1:159518/133‑1 (MQ=255)                            atatTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGAtt                                                                                                           >  1:404145/1‑128 (MQ=255)                            atatTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGAtt                                                                                                           <  2:404145/128‑1 (MQ=255)                            atatTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGAt                                                                                                      <  2:84150/133‑1 (MQ=255)                            atatTCGGAATCCCCGGAAAACGCGCGGATTGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGAt                                                                                                      <  1:247566/133‑1 (MQ=255)                                      tCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAg                                                                                            >  1:422257/1‑133 (MQ=255)                                      tCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAg                                                                                            >  2:420451/1‑133 (MQ=255)                                       ccccTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAgg                                                                                           >  1:61644/1‑133 (MQ=255)                                        cccTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGc                                                                                          <  2:70163/133‑1 (MQ=255)                                        cccTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTACTGAATCAGATGTACATTGAGGc                                                                                          >  1:130636/1‑133 (MQ=255)                                        cccTGAAAACCCGCGTATAGCGCCCGTCTTCAAATAGACATCCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGc                                                                                          <  2:130636/133‑1 (MQ=255)                                               aaCTCGCGGATAGCGCCAGTCTTCGCATAGACCTTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACt                                                                                   <  2:224456/133‑1 (MQ=255)                                               aaCTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACt                                                                                   <  2:393743/133‑1 (MQ=255)                                                           gcgcCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGt                                                                       <  2:99476/133‑1 (MQ=255)                                                           gcgcCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGt                                                                       <  2:46392/133‑1 (MQ=255)                                                                aGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCAtct                                                                  <  2:422257/133‑1 (MQ=255)                                                                      cacaTAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCt                                                            >  2:483274/1‑133 (MQ=255)                                                                       acaTAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATGCCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCtg                                                           <  1:57006/133‑1 (MQ=255)                                                                            gACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGc                                                      <  2:356929/133‑1 (MQ=255)                                                                            gACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGc                                                      <  1:486747/133‑1 (MQ=255)                                                                            gACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGc                                                      <  1:420451/133‑1 (MQ=255)                                                                             aCATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCt                                                     <  2:347191/133‑1 (MQ=255)                                                                                ttCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGGCTTTATAGTCAGCTCATTCTGGTGGCtgta                                                  >  1:226100/1‑133 (MQ=255)                                                                                 tCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGTAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCt                                                     >  2:125695/1‑129 (MQ=255)                                                                                 tCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGTAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCt                                                     <  1:125695/129‑1 (MQ=255)                                                                                               ccTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTGCTCGCCTTCATAGTCAGCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTg                                   <  2:277285/133‑1 (MQ=255)                                                                                               ccTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTg                                   <  2:56966/133‑1 (MQ=255)                                                                                               ccTGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTg                                   <  2:126556/133‑1 (MQ=255)                                                                                                 tGCGGTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCg                                 <  2:433930/133‑1 (MQ=255)                                                                                                 tGCGGTGACGTCATAGCCGAGTATCATGCTTGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCg                                 <  1:219551/133‑1 (MQ=255)                                                                                                    ggTGATGTCGGACCCGAGTATCATTCTGGCACG‑CCCAGCAGGGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGct                              <  2:240615/133‑1 (MQ=255)                                                                                                    ggTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGct                              <  1:30293/133‑1 (MQ=255)                                                                                                    ggTGATGTCATACCCCAGTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGct                              <  1:483274/133‑1 (MQ=255)                                                                                                                     gTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATaa             >  1:71473/1‑133 (MQ=255)                                                                                                                     gTATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTAATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATaa             >  2:114088/1‑133 (MQ=255)                                                                                                                      tATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAAc            <  1:7387/133‑1 (MQ=255)                                                                                                                      tATCATGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAAc            <  1:514758/133‑1 (MQ=255)                                                                                                                           tGCTGGCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTACGGCTCTATATAAAACCCATAACCAGTa       >  2:214363/1‑133 (MQ=255)                                                                                                                                gCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGa  <  2:71473/133‑1 (MQ=255)                                                                                                                                gCACG‑CCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGCCTCTATAGAGAGCCGATAACCAGTAGGGGa  >  1:148984/1‑133 (MQ=255)                                                                                                                                     |                                                                                                                                 GTACTTCTCCCGGGAGTTGTTCAACAGATATTCGGTATCCCCTGAAAACTCGCGGATAGCGCCAGTCTTCACATAGACATTCAGCTGGCTGTTGCCTGCGGTGATGTCATACCCCAGTATCATGCTGGCACGCCCCAGCAGCGATTCGTAGTGATTCAGATGTATATTGAGGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGA  >  NC_000913/1253232‑1253492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑