Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,592	(A)8→7	coding (349/429 nt)	yqaA ←	DedA family protein YqaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,585	0	A	.	100.0%	159.0 / NA	38	coding (356/429 nt)	yqaA	DedA family protein YqaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (24/14);  total (24/14)

TTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCC  >  NC_000913/2816465‑2816702                                                                                                                         |                                                                                                                      ttttAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTAc                                                                                                          <  2:116639/133‑1 (MQ=255)                     aTGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGca                                                                                      <  1:497051/133‑1 (MQ=255)                     aTGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGca                                                                                      >  2:497051/1‑133 (MQ=255)                         tGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGcatcca                                                                                  >  1:30075/1‑133 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATg                                                                                          >  1:266894/1‑123 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATg                                                                                          <  2:266894/123‑1 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGcatccatc                                                                                >  1:268646/1‑133 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGcatccatc                                                                                >  2:315216/1‑133 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGcatccatc                                                                                >  2:288054/1‑133 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGcacccatc                                                                                >  1:91069/1‑133 (MQ=255)                           aCCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGAAC‑AAAAAAAGATTACCCGTCCCCACTAGATCCGcatccatc                                                                                >  2:200355/1‑133 (MQ=255)                                     ccggCCTACAATTACGGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AACAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAg                                                                      >  2:493986/3‑133 (MQ=255)                                     cAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGCAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAg                                                                      >  1:492081/1‑133 (MQ=255)                                      aGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGa                                                                     >  1:1045/1‑133 (MQ=255)                                       ggCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGac                                                                    >  1:504598/1‑133 (MQ=255)                                        gCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGaca                                                                   <  2:1045/133‑1 (MQ=255)                                             cAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTa                                                              >  2:509992/1‑133 (MQ=255)                                                  aGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCg                                                         >  2:386462/1‑133 (MQ=255)                                                    tGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCAACCATCCCCCTACCACACACAGCAAATCGcc                                                       >  1:230688/1‑133 (MQ=255)                                                       caccacATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCACCCATCCCGCTAACAGACACAGTAAATCGCCAAc                                                    <  2:91069/133‑1 (MQ=255)                                                        accacATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAAcc                                                   >  1:316639/1‑133 (MQ=255)                                                          cacaTCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCACCCCACTAACAGACACAGTAAAACGCCAACCAc                                                 >  2:196742/1‑133 (MQ=255)                                                            catcatGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACgg                                               <  2:426453/133‑1 (MQ=255)                                                                atGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCAt                                           <  2:476033/133‑1 (MQ=255)                                                                atGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCAt                                           <  1:29208/133‑1 (MQ=255)                                                                atGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCAt                                           <  2:316639/133‑1 (MQ=255)                                                                 tGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAGCCACGGGCATc                                          <  2:492081/133‑1 (MQ=255)                                                                 tGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATc                                          <  1:288054/133‑1 (MQ=255)                                                                    ccTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAg                                       >  1:225424/1‑133 (MQ=255)                                                                    ccTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAg                                       <  1:386462/133‑1 (MQ=255)                                                                      tGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCt                                     >  1:98408/1‑133 (MQ=255)                                                                       gAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACAGAGTAAATGGCCAACTACGGGCTTCCAGCtt                                    >  1:228890/1‑133 (MQ=255)                                                                        aaCGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATcc                                         >  2:379739/1‑127 (MQ=255)                                                                        aaCGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATcc                                         <  1:379739/127‑1 (MQ=255)                                                                                  gctgcAACTGCAACATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGt                         >  2:510448/1‑133 (MQ=255)                                                                                    tgcaactgcgccATAGCGTAACGCATAACCAAGGCAC‑AAAAGAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGa                       <  2:225424/133‑1 (MQ=255)                                                                                      caactgcaacATAGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACt                     <  2:268646/133‑1 (MQ=255)                                                                                      caactgcaacATAGCGTAACGCTTAAGCTAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACt                     <  2:228890/133‑1 (MQ=255)                                                                                                  aGCGTAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGt         >  1:24226/1‑133 (MQ=255)                                                                                                      tAACGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTca     >  1:341478/1‑133 (MQ=255)                                                                                                         cGCTTTACCAAGGCAC‑AAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTcagcc  >  1:312937/1‑133 (MQ=255)                                                                                                                         |                                                                                                                      TTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCC  >  NC_000913/2816465‑2816702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑