| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,111,500 | Δ1 bp | pseudogene (489/861 nt) | yghE ← | putative type II secretion system L‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,111,499 | 0 | C | . | 87.5% | 19.5 / ‑1.5 | 8 | pseudogene (490/861 nt) | yghE | putative type II secretion system L‑type protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (4/3); total (4/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGCGATATTTTTTCAGCGCCATCGTCACCTGAGAGCGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCGCCACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCAGAATCAGCAATATCGGCAGAATCATCACCCGCCAGCGCG > NC_000913/3111389‑3111594 | tgGGCGATTTTTTTTAAGTGCCATCGTCACATGAGAGCGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCGCC‑CCTGTTCGCTGACGCTCCATa < 1:209389/133‑1 (MQ=255) gTCACCTGAGAGCGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAg < 1:361400/133‑1 (MQ=255) agagCGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCa > 1:259826/1‑133 (MQ=255) cGTAAATTCACAATCCGCTTCTGCTCTGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCAGGCCCCGCTCCAGTGTCAGCGCAACCAGAAt > 1:181123/1‑133 (MQ=255) cGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTACATAACGTTACGACCCGCTCCACTGACAGCACAACCAGAAt > 2:194632/1‑133 (MQ=255) tCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCAGAATCAGCAat < 1:401100/133‑1 (MQ=255) tCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCAGAATCAGCAATATCGGc < 1:110765/133‑1 (MQ=255) cGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCG‑CACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCAGAATCAGCAATATCGGCAGAATCATCACCCGCTAgcgcg > 1:401431/1‑133 (MQ=255) | TGGGCGATATTTTTTCAGCGCCATCGTCACCTGAGAGCGTAAATTCACAATCCGCTTCTGCTCCGGGAACAACGTTAAGAACTGTTCCTCCGCCTGGGTGCGGCTTTGCGCCACCTGTTCGCTGACGCTCCATAACGTCACGCCCCGCTCCACTGCCAGCGCAACCAGAATCAGCAATATCGGCAGAATCATCACCCGCCAGCGCG > NC_000913/3111389‑3111594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |