Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	553,219	(T)6→5	coding (722/723 nt)	lpxH ←	UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	553,214	0	T	.	100.0%	97.1 / NA	24	intergenic (‑114/+4)	purE/lpxH	N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/8);  total (16/8)

CTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACC  >  NC_000913/553115‑553341                                                                                                    |                                                                                                                                cTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCa                                                                                               >  2:515493/1‑133 (MQ=255)  tgtgGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCAt                                                                                              <  1:462228/133‑1 (MQ=255)    tggtgCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAACATGAATAAGCTCACCGTCATcc                                                                                            >  1:182802/1‑133 (MQ=255)         cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGt                                                                                       >  2:467380/1‑133 (MQ=255)         cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGt                                                                                       >  2:439068/1‑133 (MQ=255)               tctcGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACttt                                                                                 <  1:467380/133‑1 (MQ=255)                         agagGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGaa                                                                       >  2:484461/1‑133 (MQ=255)                               cacaGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTcc                                                                 >  1:391550/1‑133 (MQ=255)                               cacaGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGGCATGCGCCGTGACTTTCACCATTGAACCTTcc                                                                 >  2:178759/1‑133 (MQ=255)                                  agagAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACCTTCACCATTGAACCTTCCGTa                                                              >  1:54576/1‑133 (MQ=255)                                        tAGCACGGAACGAGACCAAGGAAATCGGTTGCTTGGCTGTGAAATAAGAAAATTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                        <  2:161009/133‑1 (MQ=255)                                        tAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAATTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTAAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                        <  1:439068/133‑1 (MQ=255)                                        tAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                        <  2:305745/133‑1 (MQ=255)                                        tAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAg                                                        <  1:484461/133‑1 (MQ=255)                                         aGCAGGGAAGGAGAGCAAGGAAAGCGGTGGCGTGGCTGTCAAATCAGCAGTTTTGC‑GGTTTTTTAAAACGAAAAATGAATCAGCTCAACGTCACCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAgg                                                       <  2:98009/133‑1 (MQ=255)                                           cACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCa                                                     >  2:272951/1‑133 (MQ=255)                                            aCGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCAc                                                    >  1:351123/1‑133 (MQ=255)                                             cGGAAAGAGGCTAAGTAACACGGTTTCTTGGCTGTGAAATCAAAAATGTT‑CGGGGTTTTTAAACCGGAAAATAAATCCGGCCAGCGTCATCCGCCGTGACTTTCTCCATTGAACCTTCCGTATGCCAGGCAcc                                                   <  2:139395/133‑1 (MQ=255)                                                aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCTGTGACTTTCAGCATTGAAACTTGCTGATGCCAAGCACCCAg                                                >  1:139395/1‑133 (MQ=255)                                                aaaGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAg                                                >  2:434205/1‑133 (MQ=255)                                                   gagagCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAc                                             <  1:352706/133‑1 (MQ=255)                                                                      gCGTGGCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGc                          >  2:480468/1‑133 (MQ=255)                                                                          ggCTGTGAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGAt                      <  1:403766/133‑1 (MQ=255)                                                                              gtgAAATCAGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAATAGTAGGTTGCTGATTGGc                  >  1:215889/1‑133 (MQ=255)                                                                                      aGCAAAGTTGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGtt          >  1:443302/1‑133 (MQ=255)                                                                                              tGCGGG‑TTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAcc  >  1:125718/1‑133 (MQ=255)                                                                                                    |                                                                                                                                CTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACC  >  NC_000913/553115‑553341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑