| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,381,779 | (G)5→6 | pseudogene (959/969 nt) | ycjV → | putative ABC transporter ATP‑binding protein YcjV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,381,774 | 1 | . | G | 91.7% | 67.6 / 0.5 | 25 | pseudogene (954/969 nt) | ycjV | putative ABC transporter ATP‑binding protein YcjV |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (15/7); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
AGCCCATCGTGATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTT‑GGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTA > NC_000913/1381650‑1381901 | aGCCCATCATGATGGCAATACGCCCGGAAGCTATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTT‑gggg‑ggca < 1:135040/133‑4 (MQ=255) cccATCGTGATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAAGCGGTGCGGTATTGATGATCTACACCGCGGTg‑gggg‑ggcacg > 2:135040/1‑128 (MQ=255) tCGTGATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGtt > 2:10992/1‑133 (MQ=255) aTGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTgg > 1:79005/1‑133 (MQ=255) aTGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCAACACCACTGTTAGGGG‑G‑CACGGGTTAGTgg > 1:225018/1‑133 (MQ=255) gggAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTc > 2:93448/1‑133 (MQ=255) aaTACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGTAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGt < 1:335367/133‑1 (MQ=255) aTACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGAGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTg > 2:490217/1‑133 (MQ=255) gAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGt > 2:259605/1‑133 (MQ=255) gAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGt > 2:12856/1‑133 (MQ=255) tCATCCGGACGCGCAGGAGGAAAATAACATATCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGAt < 1:209997/133‑1 (MQ=255) cgcgCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCa > 2:1061/1‑133 (MQ=255) gcgcAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGGGCGATAGATGATTCTCATGCAg > 1:235266/1‑133 (MQ=255) gcAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGga < 1:1061/133‑1 (MQ=255) aaTAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCa > 1:339069/1‑133 (MQ=255) cATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTAtt > 1:440741/1‑133 (MQ=255) aTTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATAATTATCATGCATGATAAAATAGCACTATTc > 1:242549/1‑133 (MQ=255) cAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGat < 1:10434/133‑1 (MQ=255) gCGGAATTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAg > 2:338925/1‑133 (MQ=255) aaTTTATGCTCTACACCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAAc < 1:118045/133‑1 (MQ=255) acacCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAAt > 2:331784/1‑133 (MQ=255) acacCACGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAAt < 2:440741/133‑1 (MQ=255) caccacGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAAt < 1:93989/132‑1 (MQ=255) caccacGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAAt > 2:93989/1‑132 (MQ=255) acGGTT‑GGGGCG‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTGAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCt < 2:225018/133‑1 (MQ=255) cGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAACTGTCATTTGTTTGATGTAGAAACGGAAATATCAATTCGCTa > 2:174500/1‑133 (MQ=255) cGGTTGGGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTa > 2:314474/1‑133 (MQ=255) cGGTT‑GGGG‑GACACGGGTTAGTGGCCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTa < 2:242549/133‑1 (MQ=255) | AGCCCATCGTGATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCCAAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTT‑GGGG‑G‑CACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTA > NC_000913/1381650‑1381901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |