| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→9 | coding (252/516 nt) | ogt ← | methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 100.0% | 60.5 / NA | 19 | T87A (ACG→GCG) | ogt | methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/11); total (8/11) | |||||||||||
| * | NC_000913 | 1,399,978 | 2 | . | C | 94.7% | 57.8 / ‑2.9 | 19 | coding (259/516 nt) | ogt | methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (8/10); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
TCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCG > NC_000913/1399846‑1400080 || tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt > 1:389770/1‑133 (MQ=255) aTCCGTTTGTGGCACGAACGGCAGTCCCCGCGTCAGGACGGGCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTGGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑ccc < 2:250420/133‑1 (MQ=255) aTCCGTTTCCCGCACCAACGGCCCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑ccc < 1:207458/133‑1 (MQ=255) gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑ccccccc > 2:462646/1‑133 (MQ=255) tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑cccccccccg < 2:328264/133‑4 (MQ=255) gCCGCACCAACGGCACGGCCCGCGCCAGGACGGCCCAATTGCTCAGCCAGGTGGGCGTAATGGTTTATCTTTCCGCGGGGGTTACCCCATAGTGGTGTCCAGTCTTCGCCCTGAACAGGTGc‑‑‑cccccccctgt > 2:127462/1‑129 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑‑CCCCCCCGTAGc < 1:497702/133‑1 (MQ=255) aCGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTggg < 1:88634/133‑1 (MQ=255) ggCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCCCGTAGCAGTGGGa < 1:358649/133‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATCCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGt < 2:381240/133‑1 (MQ=255) acgccgcaccACGACCGCGCAATTGATCTACCAGTTGCCGGTAACCCATTACCTGCCTGCTGGAGATAGTGCGTAGTGTTTTACAGTCTTCGCGCTGAACTGGCATC‑‑CCCCCCCGTAGCAGTGGAAAGCGTAt < 2:475902/132‑1 (MQ=255) cgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCaa < 1:403005/133‑1 (MQ=255) ggACGGCCCAATTGCGCAGCCAGTGGGCTGTATTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCt < 2:192513/133‑1 (MQ=255) gCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGAt < 1:127462/133‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGAtt > 1:267627/1‑133 (MQ=255) aGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTc < 1:462646/133‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCCCGTAGCAGGGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAAcc > 1:23957/1‑133 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg > 2:495216/1‑133 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGTAAAATATTCACGAAGCTTGTCGCTTAAACCGcc < 2:408324/133‑1 (MQ=255) cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTgg > 2:276830/1‑133 (MQ=255) tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGaaa > 2:31230/1‑133 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAAt > 1:439641/1‑133 (MQ=255) gtgtTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:267627/133‑1 (MQ=255) tgtTTTCCAGACTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc > 2:18340/1‑133 (MQ=255) aCTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg > 2:357084/1‑124 (MQ=255) aCTTCGCGCTGAAATGGCGTCC‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg < 1:357084/124‑1 (MQ=255) || TCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCG > NC_000913/1399846‑1400080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |