Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,580,434	(G)5→6	coding (357/1158 nt)	ydeM ←	putative anaerobic sulfatase maturation enzyme YdeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,580,429	1	.	G	100.0%	75.5 / NA	23	Q121P (CAG→CCG)	ydeM	putative anaerobic sulfatase maturation enzyme YdeM
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (12/11);  total (12/11)

ATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATA  >  NC_000913/1580299‑1580550                                                                                                                                    |                                                                                                                          aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑gg                                                                                                                         >  2:399132/1‑133 (MQ=255) aTTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑gg                                                                                                                         >  2:369671/1‑133 (MQ=255)    aaCGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑ggggg                                                                                                                      <  1:399132/133‑1 (MQ=255)       gTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑ggggggcc                                                                                                                   >  2:17539/1‑130 (MQ=255)          attaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCatc                                                                                                                 <  1:15676/133‑1 (MQ=255)          attaATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCatc                                                                                                                 >  1:266908/1‑133 (MQ=255)                     ttAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATac                                                                                                      >  1:71699/1‑133 (MQ=255)                                     tACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATg                                                                                      >  2:323780/1‑133 (MQ=255)                                       cTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGtt                                                                                    >  1:454134/1‑133 (MQ=255)                                                aTTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGaa                                                                           <  2:111049/133‑1 (MQ=255)                                                   tCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAgg                                                                        <  2:68979/133‑1 (MQ=255)                                                   tCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAgg                                                                        <  1:298527/133‑1 (MQ=255)                                                     aGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGca                                                                      <  2:263279/133‑1 (MQ=255)                                                       aCGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGcaca                                                                    <  1:40136/133‑1 (MQ=255)                                                       aCGCTCGATGGCTGCGATCACTTTTGCAAAAGCACCGTTACGTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGcaca                                                                    <  2:171536/133‑1 (MQ=255)                                                         gCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACAcc                                                                  <  2:266908/133‑1 (MQ=255)                                                                     ctATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCa                                                      >  2:304234/1‑133 (MQ=255)                                                                                   aaaGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTc                                        <  2:292823/133‑1 (MQ=255)                                                                                                             tCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt              >  2:127541/1‑133 (MQ=255)                                                                                                             tCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt              >  2:346902/1‑133 (MQ=255)                                                                                                             tCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttt              >  2:467034/1‑133 (MQ=255)                                                                                                             tCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCAATTCGTTAGTAATGTATTAAAAATACGttt              >  1:169169/1‑133 (MQ=255)                                                                                                             tCTGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGACATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGCAATGCATTAAAAATACGttt              >  2:304601/1‑133 (MQ=255)                                                                                                               tGTAACGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGttttt            >  2:486324/1‑133 (MQ=255)                                                                                                                  aaCGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGc         >  2:301007/1‑133 (MQ=255)                                                                                                                  aaCGGTCATGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGc         <  1:369671/133‑1 (MQ=255)                                                                                                                         aTGTAACTCCTGGGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATa  <  2:12524/133‑1 (MQ=255)                                                                                                                                    |                                                                                                                          ATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTGTAACGGTCATGTAACTCCT‑GGGGGCCATCGATCGAGATACCAACCAGAAATTCATGTTCTTTGAGAAAGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATA  >  NC_000913/1580299‑1580550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑