Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,155,279	(C)5→6	coding (17/3123 nt)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,155,274	1	.	C	100.0%	50.4 / NA	16	L4F (TTA→TTC)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/7);  total (9/7)

CGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTA‑CCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGC  >  NC_000913/2155156‑2155401                                                                                                                         |                                                                                                                                 cGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAgcagca                                                                                                                    >  2:330856/1‑133 (MQ=255)       aCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCGAAAAAAAGGAGCACGCTCTTGATGCAGGTGTTACCCCCC‑GAGCAGCACAggc                                                                                                               >  1:116909/1‑133 (MQ=255)       aCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAggcg                                                                                                              >  1:437651/1‑133 (MQ=255)          gAAGGGGCCAAAGTGTAACTGGTGGAAGCCAAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGcc                                                                                                           <  1:169457/133‑1 (MQ=255)            aGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGATGTTACCCCCC‑TAGCAGCACATGCGGCCCg                                                                                                         >  1:221693/1‑133 (MQ=255)             ggggCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGt                                                                                                        <  2:479127/133‑1 (MQ=255)                 cGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTcgcg                                                                                                    <  2:437651/133‑1 (MQ=255)                               gtggAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGc                                                                                      >  1:63735/1‑133 (MQ=255)                                     gCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAACAGCACAGTCGGACCGCCGCGCCTGATTATTATGCGTCCtg                                                                                >  2:161442/1‑133 (MQ=255)                                                cACTACTCCTGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTAATATGCGACCTGTGACCACCACg                                                                     >  1:245760/1‑133 (MQ=255)                                                          gAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGAtgg                                                           >  1:115913/1‑133 (MQ=255)                                                                aaaGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTACTGTGGCCACCACGCTGCTGATGGTGGCGa                                                     >  2:126867/1‑133 (MQ=255)                                                                           ccGCGACTACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGcc                                          >  2:484663/1‑133 (MQ=255)                                                                           ccGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGcc                                          <  1:330856/133‑1 (MQ=255)                                                                                      cg‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTTCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCgg                               <  1:126867/133‑1 (MQ=255)                                                                                                      gCTCCTGATGCAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCTCCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGcc                <  1:409557/133‑1 (MQ=255)                                                                                                                cAGGTGTTACCCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGgcg      <  1:484663/133‑1 (MQ=255)                                                                                                                    tgtTA‑CCCCCTGAGCTGCACAGGGCGCCAGTCACGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGc  <  2:245760/133‑1 (MQ=255)                                                                                                                         |                                                                                                                                 CGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGAGAAAGCCACCAGCCGCGAATACGCG‑AAAAAAGGAGCACGCTCCTGATGCAGGTGTTA‑CCCCC‑GAGCAGCACAGGCGGCCCGTCGCGCCTGTTTATTATGCGTCCTGTGGCCACCACGCTGCTGATGGTGGCGATCTTACTCGCCGGGATTATCGGTTATCGCGCCCTGCCCGTTTCGGCGCTGC  >  NC_000913/2155156‑2155401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑