Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,326,925	(C)6→7	coding (817/1185 nt)	atoB →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,919	1	.	C	100.0%	37.6 / NA	12	T271P (ACC→CCC)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (7/5);  total (7/5)

GCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTA‑CCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACT  >  NC_000913/2326799‑2327031                                                                                                                          |                                                                                                                 gCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCt                                                                                                       <  2:347186/133‑1 (MQ=255)                       aaGCAGGATCAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCg                                                                                 <  1:164581/133‑1 (MQ=255)                                   tCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGcccc                                                                     >  2:100799/1‑133 (MQ=255)                                              aaCGCGTCTGGTATTCACGACGGTCCTGTCGCCCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATg                                                          <  2:167351/133‑1 (MQ=255)                                                             aaCGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTa                                           >  2:265766/1‑133 (MQ=255)                                                                  cgGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAACGTTATGCCAGCGGAGGCGTGCCACCCTCCTAGATGGTGATGGGGGCAGCACCTGc                                      >  2:154354/1‑133 (MQ=255)                                                                     tgctgcCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGAGTGCCCCCGGCAATGAGGGGTATGGTGCCAGTACCTGCCAc                                   >  2:146068/1‑133 (MQ=255)                                                                               ctGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCg                         >  1:115762/1‑133 (MQ=255)                                                                                    gATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACa                    <  1:100799/133‑1 (MQ=255)                                                                                            aagaaTCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCgg            <  2:115762/133‑1 (MQ=255)                                                                                               aaTCTGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGc         >  1:108552/1‑133 (MQ=255)                                                                                                   tGCGGCGCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCCGTACCTGCTACGCAAAAAGCGTTACGACTGTCGGGGGTGCa     >  2:181245/1‑133 (MQ=255)                                                                                                      ggcgCTGGCAGCAGGCCTTACCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACt  >  2:501599/1‑133 (MQ=255)                                                                                                                          |                                                                                                                 GCATTGCGCCCGGCCTTCGATAAAGCAGGAACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGCAGCAGGCCTTA‑CCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACT  >  NC_000913/2326799‑2327031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑