| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,462,889 | (C)9→10 | intergenic (+243/‑123) | fadL → / → yfdF | long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,462,880 | 1 | . | C | 90.9% | 61.4 / 0.6 | 22 | intergenic (+234/‑132) | fadL/yfdF | long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (8/12); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACT‑‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAA > NC_000913/2462752‑2462985 | cccTGTTGGCAGCTTGCCCGTTCAAATGAATAAGCGATATCCACAGACACAGCTGAAGGGAAAGGCATATTTTCAGACATTCTGCTCGCCCTTGCCACAGAGTTTACTTTTCTGCATTTCCATCAT‑‑ACT‑‑c‑ccc > 2:430207/2‑133 (MQ=255) gTTGGCAGCTAGCCCCTTCAAATCAATATGCGCTATCCACACACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACT‑‑c‑ccccccc < 2:136405/133‑1 (MQ=255) tGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACT‑‑c‑ccccccccc < 2:329075/133‑2 (MQ=255) cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAGCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGAT‑‑GCT‑‑c‑ccccccccctgg < 1:78325/133‑5 (MQ=255) ccTTTCAAATCAATATGCGGTATCCACAAACATACCTGAAGAGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATtgtg < 2:196766/133‑1 (MQ=255) ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACT‑‑CGCCCACCCCTGGCTATTGTgcgc > 1:196766/1‑133 (MQ=255) cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTc > 1:268852/1‑133 (MQ=255) tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc > 2:72131/1‑133 (MQ=255) gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTCCC‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAAtt > 1:119099/1‑133 (MQ=255) cacaGACAAACCTGATGGGAAAAGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 1:211235/133‑1 (MQ=255) cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 2:406386/133‑1 (MQ=255) aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTCCC‑CCCCCCCCTGGCTATTGGCCGCCCATACTCCCAAATTAAAGAttg > 1:28791/1‑131 (MQ=255) aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt < 1:72131/133‑1 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑AATC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCta > 1:474147/1‑133 (MQ=255) aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc > 1:419377/1‑133 (MQ=255) ttttCAGGCGCTCTGATCGTCCTTCTCACAGATTTTTCTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa < 2:139194/133‑1 (MQ=255) cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa < 2:474147/133‑1 (MQ=255) cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa > 2:495326/1‑133 (MQ=255) ttcTCGTCCTCCTCATAGAGTTTGCTCTTCTGCATTTCCAGCAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt < 2:247188/131‑1 (MQ=255) tGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATC‑CCC‑‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt < 2:323852/133‑1 (MQ=255) tcTCGTCCTTCTCAAAGAGTTTGCTTTTTTGCAATTCCAGGATACACC‑‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt < 2:28791/132‑1 (MQ=255) gCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑TCTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt < 1:287392/133‑1 (MQ=255) cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa < 1:495326/133‑1 (MQ=255) cGTCCTTCTCCAAGACTTTACTTTCCTGGATTTCCAGGAT‑‑ATTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg < 2:138003/133‑1 (MQ=255) cGTCCTTCCCAAAGATTTGTCTTTTCTGCAATTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg < 1:252057/133‑1 (MQ=255) ctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACTCCC‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat < 2:119099/133‑1 (MQ=255) tttACTTTTCTGCATTTCCAGGAT‑‑ACTC‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTaa > 1:34298/1‑133 (MQ=255) | GCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑‑ACT‑‑C‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAA > NC_000913/2462752‑2462985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |