Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,949,9881.T94.7% 118.1 / ‑0.5 38coding (747/1332 nt)argAN‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (22/14);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGA  >  NC_000913/2949870‑2950115
                                                                                                                        |                                                                                                                               
ctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGgcg                                                                                                                    <  2:77175/133‑1 (MQ=255)
ctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGgcg                                                                                                                    <  1:69607/133‑1 (MQ=255)
  tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCa                                                                                                                  >  1:302744/1‑133 (MQ=255)
           gTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAGCTCCGGTACGGTTCA‑CTTTTTTGCGAGGCGCAGTGAAAGcc                                                                                                         >  2:229309/1‑133 (MQ=255)
             cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTg                                                                                                       >  2:48859/1‑133 (MQ=255)
             cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTg                                                                                                       >  1:359961/1‑133 (MQ=255)
             cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTg                                                                                                       >  2:275022/1‑133 (MQ=255)
             cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTg                                                                                                       >  2:255918/1‑133 (MQ=255)
                       gacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgcggc                                                                                             >  1:314374/1‑133 (MQ=255)
                       gacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAgcggc                                                                                             >  2:12149/1‑133 (MQ=255)
                            tGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcg                                                                                        >  2:384843/1‑133 (MQ=255)
                                   gTCTCCGACCTTTTCCCTCACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGt                                                                                 <  1:229309/133‑1 (MQ=255)
                                      tcCGATCTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                              >  1:488119/1‑133 (MQ=255)
                                      tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                              >  1:28127/1‑133 (MQ=255)
                                      tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                              >  1:509029/1‑133 (MQ=255)
                                        cGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGAGCAGTGAATGCCGGCCGCAGCGGCGGGCGTAGCTGTCAttt                                                                            >  2:146917/1‑133 (MQ=255)
                                           aCTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                         <  1:84094/133‑1 (MQ=255)
                                               ttCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAACGCGATTACAACTCCGGTACGGTGCG‑C‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCACAGAGGGCCGCGTCGCGGTCAAGTAATAAgct                                                                    >  2:240843/1‑131 (MQ=255)
                                                         aagcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAg                                                           <  2:392598/133‑1 (MQ=255)
                                                                  cgcgGGTATAAGCCCCGGAAGATAAAGGCGTTTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                  <  1:172457/133‑1 (MQ=255)
                                                                  cgcgGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                  <  1:48859/133‑1 (MQ=255)
                                                                                 aGGAAGAGAAAGGCGATTACAACTCTGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAgttgt                                   >  2:352634/1‑133 (MQ=255)
                                                                                         aaaGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGTGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                           >  2:282419/1‑133 (MQ=255)
                                                                                         aaaGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                           >  2:319091/1‑133 (MQ=255)
                                                                                         aaaGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGAGGGCGCGCAGTTGCAAGAGTTGTTCTCAcgc                           >  1:165429/1‑133 (MQ=255)
                                                                                          aaGGCGATTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgcg                          <  1:275022/133‑1 (MQ=255)
                                                                                                aTTACAACTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTa                    <  2:488119/133‑1 (MQ=255)
                                                                                                       cTCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTAc             >  2:50971/1‑133 (MQ=255)
                                                                                                        tCCGGTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg            <  2:314374/133‑1 (MQ=255)
                                                                                                           ggTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAg         >  2:388725/1‑133 (MQ=255)
                                                                                                            gTACGGTGCGAT‑TTTTTGCGTGGCTCAGTGAAGGCCTGCCGTAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  2:217208/133‑1 (MQ=255)
                                                                                                            gTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:464537/133‑1 (MQ=255)
                                                                                                            gTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  2:428601/133‑1 (MQ=255)
                                                                                                            gTACGGTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        <  1:50971/133‑1 (MQ=255)
                                                                                                                 gTGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATtgtg   >  2:4834/1‑133 (MQ=255)
                                                                                                                  tGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTGCGCAGa        <  1:35915/127‑1 (MQ=255)
                                                                                                                  tGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa        >  2:35915/1‑127 (MQ=255)
                                                                                                                  tGCG‑CTTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGa  >  2:482181/1‑133 (MQ=255)
                                                                                                                        |                                                                                                                               
CTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCG‑C‑TTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGA  >  NC_000913/2949870‑2950115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑