| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,177,509 | (C)6→7 | coding (402/630 nt) | nudF ← | ADP‑sugar pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,177,503 | 1 | . | C | 90.0% | 26.1 / ‑2.8 | 10 | coding (408/630 nt) | nudF | ADP‑sugar pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (7/2); total (8/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCC > NC_000913/3177378‑3177622 | tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑ccccccc > 1:360936/1‑132 (MQ=255)tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑ccccccc > 1:83293/1‑132 (MQ=255) tGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATCGACGAACCCTCCCTGGT‑GC‑CCCCCCGGGCt > 1:205576/1‑133 (MQ=255) tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAAAGCTCACTGGTGG‑‑CCGCCCGGGGTTGCCAgg > 1:139190/1‑133 (MQ=255) aCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTg > 1:296569/1‑133 (MQ=255) gcgAATATTTTCGTTTTCTTCACCCAGACCCTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt < 1:151394/133‑1 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt > 1:291539/1‑133 (MQ=255) gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGt < 2:291539/133‑1 (MQ=255) ttttCGTTTTTATCACCCAGACCGTGAATACCCCTTGCGGTCGGGGGGTCCCCTTCCCCCACCATAATTGACGAACGCCCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg < 2:205576/131‑1 (MQ=255) tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCAGCCGCTGCAATCGGTTCGCGAc > 1:142070/1‑133 (MQ=255) aCGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca > 1:16193/1‑133 (MQ=255) aCGAACGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca > 2:286540/1‑133 (MQ=255) aCGCTCACTGGT‑GC‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATccc > 1:70663/1‑133 (MQ=255) | TGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCC > NC_000913/3177378‑3177622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |