| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,379,266 | (C)7→8 | coding (732/1128 nt) | zapE ← | cell division factor ZapE with ATPase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,379,259 | 1 | . | C | 92.0% | 78.3 / 0.3 | 25 | coding (739/1128 nt) | zapE | cell division factor ZapE with ATPase activity |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (10/13); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTA > NC_000913/3379129‑3379387 | gcAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑cc > 2:474330/1‑133 (MQ=255) gTCATGCTCACTGCGTGCCTCGACGCACGGTGTAGTAAAAGAGACCGCCAGCGTATGGTTCTCAACGCCCATTGTTGCTAACGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑ccccccccgc < 1:193812/133‑4 (MQ=255) gCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAgcg < 1:291839/133‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAgcgc < 2:506499/133‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAgcgc < 1:497230/133‑1 (MQ=255) cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAgcgc < 1:410164/133‑1 (MQ=255) aCTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCaa > 2:118549/1‑133 (MQ=255) tGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAAcc < 1:70461/133‑1 (MQ=255) gCGGGCGTAGACGCACAGTGTAATAAAAGAAACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCa < 1:241272/133‑1 (MQ=255) gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa > 2:505574/1‑133 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCGGCGCCAACAATAGTTTATCCATTTGTGCCCAGGTTTCATCg > 2:136348/1‑133 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACGATAGTTTATCCATTTGAGCCCGGGTTTCATCg > 2:143872/1‑133 (MQ=255) aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACGATAATGTATCCATTTGGGCCCAGGTTTCATCg > 2:235889/1‑133 (MQ=255) ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt > 2:492513/1‑133 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg > 1:498106/1‑133 (MQ=255) cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAATTTATCCATTTCCGCCCGGGTATCATCGTg > 1:194018/1‑133 (MQ=255) tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa < 1:474330/133‑1 (MQ=255) tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc < 1:505574/133‑1 (MQ=255) cccTTTGTTGATATGTGCCGATGGCTGAATTCTAACGTCGGCGATTTTCCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc < 2:147684/133‑1 (MQ=255) ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg < 1:492513/133‑1 (MQ=255) cGATGGTTCATTTCTGACCTCGCTGAATTTCCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc < 1:136348/133‑1 (MQ=255) agATGGTTGATTTCTGACCTCGCTGAATTTCCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc < 1:191653/132‑1 (MQ=255) gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTCC‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCa < 2:483769/133‑1 (MQ=255) tGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGa < 2:498106/133‑1 (MQ=255) aCGTCGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGGCTGAGTGAGTGTACGCTGACGGTAATCAacac > 2:181078/1‑133 (MQ=255) cGGTGAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGc > 1:439676/1‑133 (MQ=255) gAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 1:107365/133‑1 (MQ=255) gAATTTTCTCGTT‑TCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc < 1:118549/133‑1 (MQ=255) cGTT‑T‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTa < 2:483132/133‑1 (MQ=255) | GCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTA > NC_000913/3379129‑3379387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |