| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,931,183 | (G)7→8 | intergenic (‑90/‑133) | ravA ← / → kup | regulatory ATPase RavA/K(+):H(+) symporter Kup |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,931,176 | 1 | . | G | 96.7% | 90.4 / ‑3.0 | 30 | intergenic (‑83/‑140) | ravA/kup | regulatory ATPase RavA/K(+):H(+) symporter Kup |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (15/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
AATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACG > NC_000913/3931061‑3931303 | aaTTCTTTACGCTAATAATTGATGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCt < 1:134615/133‑1 (MQ=255) aTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCtt < 1:498930/133‑1 (MQ=255) tttCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAg < 2:7279/133‑1 (MQ=255) gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg > 1:128636/1‑128 (MQ=255) gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg < 2:128636/128‑1 (MQ=255) gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCa > 2:305796/1‑133 (MQ=255) aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCAttt > 2:25247/1‑133 (MQ=255) aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTGTCTTTGAGCCAGGTTAATGTGCCCGCAttt > 1:167263/1‑133 (MQ=255) aaTGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGt > 2:77700/1‑133 (MQ=255) aaGTGGCGTCCTTTCGTCAAAAGTTCTGCGGAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGa > 2:95366/1‑133 (MQ=255) ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtttt < 1:77700/133‑1 (MQ=255) cGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGc > 1:461903/1‑133 (MQ=255) cGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGCTAATGTGTCCGCATTTAGGAGTACGATTTTGc > 2:217692/1‑133 (MQ=255) tCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCg > 2:112690/1‑133 (MQ=255) ccTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt < 1:305796/133‑1 (MQ=255) tttCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa < 2:458500/133‑1 (MQ=255) tttCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa < 2:469740/133‑1 (MQ=255) ttCGTCAAAACTTCTACGTAAATGACGGATATCGCCGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTTTTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTA‑GAGTACGATTTTGCCGTTAAt < 2:141202/133‑1 (MQ=255) cTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCAGTGAATCGTACATACtgt > 1:238879/1‑133 (MQ=255) aTTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCtttt > 1:25453/1‑133 (MQ=255) gAGTATAGAGCTTTCCTGCTGGTTTCGAGAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtatg < 2:238879/133‑1 (MQ=255) aGTATAGACGTTTCTTGGTGGTGGCTAAAATAGTCTCAAAGGGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCATTAATCGGGCATACGGTGCGCCTTTTtgtg > 1:234461/1‑133 (MQ=255) ataGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCa < 1:404502/133‑1 (MQ=255) taGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCa > 2:94983/1‑133 (MQ=255) gTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGa < 1:25247/133‑1 (MQ=255) gCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGca < 1:112690/133‑1 (MQ=255) tAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATAttt < 1:312194/133‑1 (MQ=255) tctcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGGACGCATTTCTTATTTCaa > 1:201875/1‑133 (MQ=255) tctcAAAG‑GGGGGGGCATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCaa > 1:19397/1‑133 (MQ=255) tcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACg < 1:79850/133‑1 (MQ=255) | AATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACG > NC_000913/3931061‑3931303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |