| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,060,843 | (G)6→7 | coding (397/711 nt) | yihL → | putative transcriptional regulator YihL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,060,837 | 1 | . | G | 96.9% | 103.9 / ‑3.1 | 32 | coding (391/711 nt) | yihL | putative transcriptional regulator YihL |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (17/14); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
GCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑G‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAA > NC_000913/4060719‑4060963 | gCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGa < 2:353801/133‑1 (MQ=255)gCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGa < 1:394199/133‑1 (MQ=255) gggCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGa > 2:483186/1‑133 (MQ=255) gCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGTATCACCGCCT‑GCGGGGGCGCTTGAAGGACa > 1:161998/1‑133 (MQ=255) gagaGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATa > 1:55367/1‑133 (MQ=255) gagaGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATa < 1:483186/133‑1 (MQ=255) gggTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCAc < 2:127605/133‑1 (MQ=255) ggTTTACCGAGAAAACCCGTACCACCGAAATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg < 2:181541/133‑1 (MQ=255) ggTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg < 1:293985/133‑1 (MQ=255) ggTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg < 1:400432/133‑1 (MQ=255) ggTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg < 1:278403/133‑1 (MQ=255) gTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGa < 1:26421/133‑1 (MQ=255) tttACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGaa < 2:69159/133‑1 (MQ=255) ttACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGaaa > 1:495367/1‑133 (MQ=255) tACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAc > 1:420105/1‑133 (MQ=255) aCCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACa < 1:448168/133‑1 (MQ=255) cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCtt < 2:55367/133‑1 (MQ=255) agaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCt > 1:42532/1‑123 (MQ=255) agaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCt < 2:42532/123‑1 (MQ=255) aCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATAAATTAATCATGACGTAGCTCCGTGCTTTAtt > 1:238990/1‑133 (MQ=255) tCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaac < 1:344650/133‑1 (MQ=255) cgcgCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGGAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaaca > 2:123765/1‑133 (MQ=255) aGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTc > 2:8262/1‑133 (MQ=255) aCGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCAttt > 2:285220/1‑133 (MQ=255) cGCCATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCAtttt > 2:432529/1‑133 (MQ=255) ccATCAACTGAACTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGACTTTATTGAACAACTTGAAAACCACTCATTTTCt > 2:116813/1‑133 (MQ=255) aaCTCTATCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTggg > 2:414130/1‑133 (MQ=255) tCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTg > 2:33860/1‑133 (MQ=255) tCGCATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTg > 2:74526/1‑133 (MQ=255) cATCACCGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTa > 1:384248/1‑133 (MQ=255) ccGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCaaa > 1:93201/1‑133 (MQ=255) ccGGCTGG‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCaaa > 1:128494/1‑133 (MQ=255) | GCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑G‑GGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAA > NC_000913/4060719‑4060963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |