Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	546,454	(A)7→8	intergenic (‑91/‑226)	allD ← / → fdrA	ureidoglycolate dehydrogenase/putative acyl‑CoA synthetase FdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	546,447	1	.	A	89.3%	81.7 / 3.5	28	intergenic (‑84/‑233)	allD/fdrA	ureidoglycolate dehydrogenase/putative acyl‑CoA synthetase FdrA
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base A (16/9);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

AGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGC‑AAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAATTCCT  >  NC_000913/546315‑546576                                                                                                                                      |                                                                                                                                    agagTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGc                                                                                                                                      <  2:292452/133‑1 (MQ=255)    gTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGC‑aaa                                                                                                                                  >  1:486315/1‑133 (MQ=255)        aTTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGGTTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATTTGGATTATGTGATCCAGACAGGC‑aaa‑aaaa                                                                                                                             >  2:101796/1‑133 (MQ=255)                   gCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGa                                                                                                                  <  1:124395/133‑1 (MQ=255)                                 tCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TGTAGATATAATTTAATTGATAAt                                                                                                    >  1:237759/1‑133 (MQ=255)                                     gACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGc                                                                                                >  1:364432/1‑133 (MQ=255)                                      aCTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCt                                                                                               <  2:280040/133‑1 (MQ=255)                                      aCTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATCTATTTGATAATCCGCt                                                                                               <  1:82654/133‑1 (MQ=255)                                          aTTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑AATAGTTAGAATTTATTTGATAATCCGCTCACt                                                                                           >  2:170370/1‑133 (MQ=255)                                                   tGTCTTCCTTTTTAAGGAGGGTTTGAAGCACGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTg                                                                                  <  1:211258/133‑1 (MQ=255)                                                   tGTCTTCCTCATTAATGACGGTGTAAAGCATGTCTGCAATTTACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTg                                                                                  <  2:237759/133‑1 (MQ=255)                                                    gTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGACAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGa                                                                                 >  1:367194/1‑133 (MQ=255)                                                             tttAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATTATCCGCTCACTTTTGACCTGAATTTTaaaa                                                                        >  1:174863/1‑133 (MQ=255)                                                                    acgGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTAGATAATCCACTCACTTTTAACCTGAATTTTAAaacaacaac                                                                 >  2:171469/1‑133 (MQ=255)                                                                                           tAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTaa                                          <  2:364432/133‑1 (MQ=255)                                                                                                tACAGGGATAATATCTGGTTTAGGTGATCCTGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGcc                                     <  1:171469/133‑1 (MQ=255)                                                                                                 aCAGGGAAAATATCTGGATTATGTGATCCATACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCt                                    >  2:411671/1‑133 (MQ=255)                                                                                                 aCAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCt                                    >  2:62391/1‑133 (MQ=255)                                                                                                 aCAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCt                                    >  1:267077/1‑133 (MQ=255)                                                                                                 aCAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCt                                    >  1:257710/1‑133 (MQ=255)                                                                                                  cAGGGAAAATATCTGGATTATGTGATCCAGACAGGC‑AAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAATAAATGATGAGTAATGGCAtgt                                  >  2:476985/1‑132 (MQ=255)                                                                                                               tGGATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGaa                      >  1:17447/1‑133 (MQ=255)                                                                                                                 gATTATGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAAtt                    >  1:299388/1‑133 (MQ=255)                                                                                                                     aTGTGATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCttt                >  1:479247/1‑133 (MQ=255)                                                                                                                      tgtgATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCtttt               >  2:480430/1‑133 (MQ=255)                                                                                                                       gtgATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTg              <  2:17447/133‑1 (MQ=255)                                                                                                                       gtgATCCAGACAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTg              <  2:299388/133‑1 (MQ=255)                                                                                                                                aCAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAAtt     >  1:446214/1‑133 (MQ=255)                                                                                                                                aCAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAAtt     >  1:290529/1‑133 (MQ=255)                                                                                                                                 cAGGCAAAA‑AAAA‑TATAGTTAGAATTTATTTGCTAATCCGCTCACTTTTAACCTGATTTTTAAAATAACAACGCTTATTAAAAAATAATGAGTATTTGCCTGGTGGTTATTTGAATTCTTTTGTTAATAATTc    <  2:176487/133‑1 (MQ=255)                                                                                                                                 cAGGC‑AAATAAAA‑TAGAGTTAGAATTTATTCGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAATTc    <  1:171302/133‑1 (MQ=255)                                                                                                                                   ggC‑AAA‑AAAAGTATAGTTAGAATTTATTTGATTATCCGCTCGCTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAATTCCt  <  2:174863/133‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    AGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGC‑AAA‑AAAA‑TATAGTTAGAATTTATTTGATAATCCGCTCACTTTTAACCTGATTTTTAAAACAACAACGCTTATTAAAAAATAATGAGTAATAGCCTGGTGGTTATTTGAATTCTTTTGTTAATAATTCCT  >  NC_000913/546315‑546576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑