| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 766,367 | (C)6→7 | coding (384/1977 nt) | mngA → | 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 766,361 | 1 | . | C | 83.3% | 45.3 / 4.7 | 18 | coding (378/1977 nt) | mngA | 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (5/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑C‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGC > NC_000913/766232‑766485 | ggCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑c‑cc > 1:310393/1‑133 (MQ=255) tgCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑c‑cccccca < 1:29367/133‑3 (MQ=255) ccGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑C‑CCCCCAATg > 1:450468/1‑133 (MQ=255) cGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑c‑ccccccaatg > 1:75402/1‑128 (MQ=255) gCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGa < 2:75402/133‑1 (MQ=255) cATGGCAACACTGCTGCGCTAAAAGAAGCGTCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGaa < 1:130052/133‑1 (MQ=255) tGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGc < 1:121011/133‑1 (MQ=255) ctgctgCGCTAAAAGGAGTGGCCTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGtacta < 1:147828/133‑1 (MQ=255) gTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATg < 2:93026/133‑1 (MQ=255) gcggcgTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑C‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGa < 2:450468/133‑1 (MQ=255) cAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGc > 1:416050/1‑133 (MQ=255) cGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGTCAGCGCTAACCTCGTGCCTTGAGGatgatg > 1:244958/1‑133 (MQ=255) ttCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑C‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGAt > 1:488435/1‑133 (MQ=255) cAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGAtt > 1:332388/1‑133 (MQ=255) gTGGGAAGGCTTTGATGGCCCGGACGCATTTGTTTTAGTGGAGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCg < 2:152748/133‑1 (MQ=255) gTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCg < 2:332388/133‑1 (MQ=255) ggAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGgc < 2:416050/133‑1 (MQ=255) ggAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGcgac < 1:439711/133‑1 (MQ=255) ttGATTTAGTGGTGCTGCTGGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCtg > 2:113448/1‑133 (MQ=255) gtgCTGCTGGCGATT‑C‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGctct < 2:360131/133‑1 (MQ=255) tgctgcTGACGATT‑CGCCTCCAATGAAGCGCGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGctct < 1:219323/133‑1 (MQ=255) ctgctgGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAATCGGCGAGGACGCCGGATGAGTTGCTCTCg > 1:170366/1‑133 (MQ=255) tgGCGATTCC‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGgcgc < 2:310393/133‑1 (MQ=255) | GGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATT‑C‑CCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGC > NC_000913/766232‑766485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |