Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,933	T→C	T59A (ACC→GCC)	ycgX ←	uncharacterized protein YcgX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,933	0	T	C	100.0%	113.6 / NA	35	T59A (ACC→GCC)	ycgX	uncharacterized protein YcgX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

CGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGTTTTGCT  >  NC_000913/1212803‑1213058                                                                                                                                   |                                                                                                                              cGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGTTCGTACTAACTGTTGACAggttcctggctc                                                                                                                             >  1:252985/1‑133 (MQ=255) cGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAggttcctggctc                                                                                                                             >  2:270064/1‑133 (MQ=255) cGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAggttcctggctc                                                                                                                             >  2:458416/1‑133 (MQ=255) cGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGATCGTACTCACTATTAACAggtacctggctc                                                                                                                             >  1:198682/1‑133 (MQ=255)      aaCGCCCGTGCCTGCTAGCGTTAAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCCCCTCtt                                                                                                                        <  1:240128/133‑1 (MQ=255)              cgcCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACaa                                                                                                                <  1:300147/133‑1 (MQ=255)                ccTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACaaaa                                                                                                              <  1:458416/133‑1 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCg                                                                                                   >  2:438993/1‑131 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCg                                                                                                   <  1:438993/131‑1 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTg                                                                                                 <  2:382173/133‑1 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTg                                                                                                 <  1:270064/133‑1 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTg                                                                                                 >  1:177324/1‑133 (MQ=255)                             aaCAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTg                                                                                                 <  1:294744/133‑1 (MQ=255)                                  tATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGtaa                                                                                            >  2:94439/1‑133 (MQ=255)                                          aaaTGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTg                                                                                    <  2:1462/133‑1 (MQ=255)                                            aTGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGAt                                                                                  >  2:929/1‑133 (MQ=255)                                                       ccGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAg                                                                       >  2:5234/1‑133 (MQ=255)                                                        ctcAAATATGTAGCACTGCGGCCCATTTCATTTTAGCAGGGGTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGc                                                                      <  2:252985/131‑1 (MQ=255)                                                           aaaTATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAg                                                                   <  2:438630/133‑1 (MQ=255)                                                             atatGTAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGat                                                                 <  1:286772/133‑1 (MQ=255)                                                               atGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGataa                                                               <  2:116124/133‑1 (MQ=255)                                                                     cAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAaatg                                                         <  2:406233/133‑1 (MQ=255)                                                                      aaTGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAaatgt                                                        <  2:387291/133‑1 (MQ=255)                                                                       aTGCGGCCTCGTTTACTTTAGCAGAGTTCTTGTTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAaatgta                                                       <  2:152747/133‑1 (MQ=255)                                                                       aTGCGGCCTCCTTTAGTTTAGCAGGGTTCTTGCTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAaatgta                                                       <  2:178613/133‑1 (MQ=255)                                                                                ctTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAAt                                              >  2:260353/1‑133 (MQ=255)                                                                                       tttaGCAGTGTTCTTTGTGGTATTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGa                                       <  1:183886/133‑1 (MQ=255)                                                                                       tttaGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGa                                       <  1:94439/133‑1 (MQ=255)                                                                                       tttaGCAGGGTTCTTAGTCGTACTGACTGTTATCAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGtta                                       <  2:177324/133‑3 (MQ=255)                                                                                       tgtaGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGa                                       <  2:323069/131‑1 (MQ=255)                                                                                         taGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAAATAATAAGTGTCATGTGCAATACGGTGATg                                     >  1:235462/1‑133 (MQ=255)                                                                                               ggTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTaa                               >  1:450297/1‑133 (MQ=255)                                                                                                gTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTATCCAGATAATAAAGGTAATGTGCAATCCGGTCATGTTTTAAt                              >  2:186470/1‑133 (MQ=255)                                                                                                       gTCGTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAAt                       <  1:32908/133‑1 (MQ=255)                                                                                                          gTACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATaaa                    >  1:372115/1‑133 (MQ=255)                                                                                                           tACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATaaaa                   >  2:455542/1‑133 (MQ=255)                                                                                                           tACTAACTTTTAACAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGAGCAATACGGTGATGTTTTAATACTGAATaaaa                   >  2:204489/1‑133 (MQ=255)                                                                                                                      aaCAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGt        >  1:405200/1‑133 (MQ=255)                                                                                                                      aaCAGGTTCCTGGCTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGt        >  2:79357/1‑133 (MQ=255)                                                                                                                          ggttcctggctcctCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGTTTTg    <  1:79357/133‑1 (MQ=255)                                                                                                                            ttcctggctcctCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGTTTTGct  <  2:264428/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                              CGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGTTATCCGTAGCCAGATAATAAATGTAATGTGCAATACGGTGATGTTTTAATTCTGAATAAAACCAGGAGAAGTTTTGCT  >  NC_000913/1212803‑1213058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑