| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,240,425 | A→G | K56E (AAA→GAA) F549F (TTT→TTC) |
ymgM → cvrA ← |
protein YmgM putative K(+):H(+) antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,240,425 | 0 | A | G | 95.0% | 61.4 / NA | 20 | K56E (AAA→GAA) F549F (TTT→TTC) | ymgM cvrA | protein YmgM putative K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/7); minor base T (0/1); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCA > NC_000913/1240308‑1240551 | cAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCa < 2:268074/133‑1 (MQ=255) gATAGATGCTTCATTCAATTTACGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAAc < 1:176068/133‑1 (MQ=255) gCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGcc > 1:296060/1‑133 (MQ=255) cATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCgcgc > 2:445489/1‑133 (MQ=255) cATTAAATTTAAGATTCAGCTCCTTCTTCAGCCCCCCGTCCGCCAATCTTCAACTCTTCATTATCTTCCTTCTCGGCCGCCGTACAGATCTTCCCCGCGGACTCTTCCTGGTCGCCGACAACCGGTGCCgcgc < 2:478774/133‑1 (MQ=255) tttAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTaacaa < 2:68064/133‑1 (MQ=255) aTTCAGCTTCTTCTTGAGCCACCCATACTCCACTCTTCAACTCTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAActgctg < 2:131659/133‑1 (MQ=255) ccTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGATGCCGCGACTAACACCTGCTGGACAATTTc > 1:180111/1‑133 (MQ=255) cAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAg > 1:418280/1‑133 (MQ=255) cccGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtg > 1:48389/1‑133 (MQ=255) cccGTACGCCAATCTGCAACACTTTATTGTCTGCTTTCTCGTCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtg < 2:169349/133‑1 (MQ=255) aTCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCAc > 2:84382/1‑133 (MQ=255) aTCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAAATGCACCCAGCTTTTGCCGCTTATCAc > 2:237011/1‑133 (MQ=255) acTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATtctc > 1:484414/1‑133 (MQ=255) cATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGccc > 2:55703/1‑133 (MQ=255) tctttctCGGCCACCTTCCAGATCGTCCGGGCTAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa < 2:180111/133‑1 (MQ=255) tctcGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGcc > 1:512071/1‑133 (MQ=255) tctcGGCCACCGTCCAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTCATCACGAGATTCTCGCCCGTCGTCCAGGcc > 1:223049/1‑133 (MQ=255) cAGATCATCCCGGCGTACCCTAACTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCaa < 1:237011/133‑1 (MQ=255) cAGATCATCCCGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCaa < 1:84382/133‑1 (MQ=255) cccGGCGAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCa > 1:116642/1‑133 (MQ=255) | CAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCA > NC_000913/1240308‑1240551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |