Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,673 T→C G102G (GGT→GGC)  leuO → DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,6730TC95.0% 117.2 / ‑2.1 40G102G (GGT→GGC) leuODNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (14/24);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

GAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTC  >  NC_000913/84547‑84802
                                                                                                                              |                                                                                                                                 
gAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAGCTAGTACGAAATGATTTGCCTGGCTCAGGt                                                                                                                             >  1:250672/1‑133 (MQ=255)
gAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGt                                                                                                                             >  2:28003/1‑133 (MQ=255)
        tGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAAcc                                                                                                                     >  2:450446/1‑133 (MQ=255)
        tGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAAcc                                                                                                                     >  1:376923/1‑133 (MQ=255)
               tGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGc                                                                                                              <  1:7473/133‑1 (MQ=255)
               tGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGc                                                                                                              <  1:33083/133‑1 (MQ=255)
               tGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGc                                                                                                              <  1:31457/133‑1 (MQ=255)
                 aCGAGCTTTCAGTTCTTTATGGCCGTGGGATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAg                                                                                                            <  2:250672/133‑1 (MQ=255)
                    aGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCGTTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGa                                                                                                         >  2:127075/1‑133 (MQ=255)
                      cTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAAc                                                                                                       <  1:101130/133‑1 (MQ=255)
                       tttttGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCGTTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACg                                                                                                      <  1:127075/133‑1 (MQ=255)
                       tttttGTTCGTTATGGCCGTGGTAATCATCCGTCTGCTCGCCCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACg                                                                                                      <  2:165026/133‑1 (MQ=255)
                       tttttGTTAGTTATGGCCTTGGAATTCAACGGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACg                                                                                                      <  1:230613/133‑1 (MQ=255)
                                           ggTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGttt                                                                                  >  1:54979/1‑133 (MQ=255)
                                            gTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGTCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTg                                                                                 <  2:64373/133‑1 (MQ=255)
                                                   aCCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCg                                                                          <  2:54979/133‑1 (MQ=255)
                                                   aCCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCg                                                                          <  2:70021/133‑1 (MQ=255)
                                                   aCCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCg                                                                          >  2:359451/1‑133 (MQ=255)
                                                   aCCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCg                                                                          <  1:450446/133‑1 (MQ=255)
                                                       aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAg                                                                      >  2:291299/1‑133 (MQ=255)
                                                       aCTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAACAGTGAACATGTCTTTCATCTTTGTATTTGAAGCCCGTTAg                                                                      >  2:223769/1‑133 (MQ=255)
                                                         tGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGAc                                                                    >  2:448280/1‑133 (MQ=255)
                                                         tGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCGTTGTGTTTGCAGCCCGTTAGAc                                                                    >  1:175841/1‑133 (MQ=255)
                                                          gCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACa                                                                   >  1:279789/1‑133 (MQ=255)
                                                                    ttCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGAc                                                         <  2:128235/133‑1 (MQ=255)
                                                                    ttCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGAc                                                         >  1:128235/1‑133 (MQ=255)
                                                                         cTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGc                                                    <  1:433309/133‑1 (MQ=255)
                                                                              tGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGAtt                                               <  2:451211/133‑1 (MQ=255)
                                                                                      ttCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGTCAGCATTGTGACCTGGCAGATTTATAATca                                       >  1:205018/1‑133 (MQ=255)
                                                                                        cGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATcaca                                     <  1:396513/133‑1 (MQ=255)
                                                                                                 ttGCAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGa                            <  1:9364/133‑1 (MQ=255)
                                                                                                    cAACTAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCCCGCAGATTTATCATCACATTGTGCAGATTg                         >  1:177746/1‑133 (MQ=255)
                                                                                                       cTAGTACAAATTGAACTGCCTGGCCCAGGTTTCGAACCCGCCAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTgcgc                      <  2:205018/133‑1 (MQ=255)
                                                                                                        tAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                     <  1:16765/133‑1 (MQ=255)
                                                                                                        tAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                     <  2:102047/133‑1 (MQ=255)
                                                                                                        tAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                     <  1:291299/133‑1 (MQ=255)
                                                                                                        tAGTACAAAATGAATTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGcc                     <  1:359451/133‑1 (MQ=255)
                                                                                                                   gAATTGCCAGGCTCAGGTTTTGGACCCGCGTGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACatg          <  1:223769/133‑1 (MQ=255)
                                                                                                                     aTTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACatgtt        <  2:3343/133‑1 (MQ=255)
                                                                                                                     aTTGCCTGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACatgtt        >  1:31370/1‑133 (MQ=255)
                                                                                                                           tGGCTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTc  <  2:376923/133‑1 (MQ=255)
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GAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAATATACATGTTATGTTC  >  NC_000913/84547‑84802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑