Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,326	A→G	noncoding (3/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,326	0	A	G	100.0%	76.2 / NA	23	noncoding (3/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

AATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCG  >  NC_000913/1287202‑1287446                                                                                                                             |                                                                                                                         aaTTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGt                                                                                                                  <  1:101363/133‑1 (MQ=255)    tGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTATGGCCGCACGGAAATCCCGCCTGGGGTAAt                                                                                                               >  1:151147/1‑133 (MQ=21)      cGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTc                                                                                                             >  2:113891/1‑133 (MQ=255)        gATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACTTTCGAAGCCGATGACGGTAGATTTACTGTCTACTCGCATACGCCACTCGCGATCCCGCCCAGCGGAAAttaga                                                                                                           >  2:187474/1‑130 (MQ=16)        gATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCaa                                                                                                           >  2:106812/1‑133 (MQ=255)            cGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAAttt                                                                                                       <  1:113891/133‑1 (MQ=255)                 ggAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAgg                                                                                                  <  1:444667/133‑1 (MQ=255)                  gAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGt                                                                                                 <  2:268650/133‑1 (MQ=255)                        cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCt                                                                                           >  2:30527/1‑133 (MQ=255)                            gACTTTTGCAAGTGAAGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTgag                                                                                       <  1:195055/133‑1 (MQ=255)                                tttGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTAAGGTAATGCGTGAGAtgg                                                                                   >  1:160813/1‑133 (MQ=255)                                         gATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTggggg                                                                          >  1:40178/1‑133 (MQ=255)                                                  gggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGattat                                                                 >  1:451492/1‑133 (MQ=255)                                                   ggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGattatt                                                                <  1:30527/133‑1 (MQ=255)                                                                   cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATTCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGctcctc                                                <  2:31537/133‑1 (MQ=255)                                                                    ttCGAAGTCGCTGACAGCAGATTTTCAGACTGATCCCTTTGACCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCa                                               <  2:160813/133‑1 (MQ=255)                                                                     tCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCAc                                              >  2:407270/1‑133 (MQ=255)                                                                           tCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCg                                        <  1:106812/133‑1 (MQ=255)                                                                                gACGGCAGATCTACAGTCTGCTCCCTTTAGCCCCTCGGGAAGCCCGCGAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCg                                   <  2:151147/133‑1 (MQ=255)                                                                                                    cTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACGCTTCGGGACGTAGCATGTGGCGACGTtctc               >  1:161601/1‑133 (MQ=255)                                                                                                    cTCCCTTTGGCCGCTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTtctc               >  2:468264/1‑133 (MQ=255)                                                                                                              ccGCTGGGGACCCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGc     <  2:442138/133‑1 (MQ=255)                                                                                                                 cTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcg  >  1:348636/1‑133 (MQ=255)                                                                                                                 cTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcg  >  1:305786/1‑133 (MQ=255)                                                                                                                 cTCGGGAACCCCGCCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcg  >  2:504783/1‑133 (MQ=255)                                                                                                                             |                                                                                                                         AATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCG  >  NC_000913/1287202‑1287446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑