Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,514	T→C	intergenic (‑186/+24)	tyrV ← / ← tyrT	tRNA‑Tyr/tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,514	0	T	C	100.0%	43.2 / NA	15	intergenic (‑186/+24)	tyrV/tyrT	tRNA‑Tyr/tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/6);  total (9/6)

CGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCA  >  NC_000913/1287388‑1287620                                                                                                                               |                                                                                                           cgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACcac                                                                                                      >  2:126372/1‑133 (MQ=255)  gcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACcaca                                                                                                     >  1:320574/1‑133 (MQ=255)          cctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCtgtg                                                                                             <  1:85862/133‑1 (MQ=255)          cctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCtgtg                                                                                             <  2:451492/133‑1 (MQ=255)           ctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTgtgg                                                                                            <  2:305786/133‑1 (MQ=255)                ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAAt                                                                                       >  2:396131/1‑133 (MQ=255)                  ttCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGCGAATTa                                                                                     >  1:271865/1‑133 (MQ=255)                                        cGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACGACATCGCTATGGGGAAGTATGGTGGAGGGGAAAGGATTCGa                                                               >  2:131689/1‑133 (MQ=255)                                                            aatcgaaCGTTAGTCGAATGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCATAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg                                           <  1:187474/133‑1 (MQ=255)                                                              tcgaaCCTCAGTCGACGGCTCTCACCCTTCACCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                         <  1:131689/133‑1 (MQ=38)                                                                                   tcACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCttt                    >  1:328753/1‑133 (MQ=255)                                                                                          tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTACTCCCTTTGGCCGCt             >  1:250929/1‑133 (MQ=255)                                                                                              cGATGAGTGCGAACTCTCACAATCCCACAGAACCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGTTGCGAACCTTCGATGTCGATGACGGCAAGTTTACAGTCTGCTCCCTTTGGCCGCTCggg         <  2:161601/133‑1 (MQ=18)                                                                                                 tGAGTGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc      >  1:56073/1‑133 (MQ=255)                                                                                                     tGCAAACTTTCACAATCTCACCGAAGCTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCcca  >  1:422815/1‑133 (MQ=255)                                                                                                                               |                                                                                                           CGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCA  >  NC_000913/1287388‑1287620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑