Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,299,211	G→C	G205A (GGT→GCT)	ychE →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,299,211	0	G	C	100.0%	117.2 / NA	34	G205A (GGT→GCT)	ychE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/15);  total (19/15)

TTGTTGGGGATTGTTCCGCATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGT  >  NC_000913/1299086‑1299340                                                                                                                              |                                                                                                                                  ttgttgGGGATTGTTCCGCATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAg                                                                                                                            <  2:272668/133‑1 (MQ=255)  tgttgGGGATTGTTCCGCATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAgg                                                                                                                           <  2:125348/133‑1 (MQ=255)     tgGGGATTGTTCCGCATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGa                                                                                                                        >  2:410311/1‑133 (MQ=255)              ttCCGCATGGTACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATATATGGTTCCTGCTATTCAGGGGATTTTCCCCg                                                                                                               >  2:187963/1‑133 (MQ=255)                   cATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTg                                                                                                          >  1:207676/1‑133 (MQ=255)                                            ttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGaaa                                                                                 <  2:266275/133‑1 (MQ=255)                                            ttACGCCACACCGGCATCTACGAGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGaaa                                                                                 <  1:187963/133‑1 (MQ=255)                                                 ccAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAg                                                                            <  2:457346/133‑1 (MQ=255)                                                                                     gCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACt                                        <  2:489144/133‑1 (MQ=255)                                                                                      cTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACtt                                       >  1:72213/1‑133 (MQ=255)                                                                                      cTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACtt                                       >  2:350529/1‑133 (MQ=255)                                                                                      cTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACtt                                       >  1:50050/1‑133 (MQ=255)                                                                                        aTTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTg                                     >  1:368253/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGCGCTGCCATCCTCCGTTAACTTCGTCATTATCTTTACTTTGt                                    >  2:151015/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                    >  1:435621/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                    >  1:437485/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                    >  1:8729/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                    >  2:461167/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                    >  2:367203/1‑133 (MQ=255)                                                                                         ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCCCCGTTTAATTCTTCATTATTTTTACTTTGt                                    >  1:252638/1‑133 (MQ=255)                                                                                              gATGGCATTGGGGATCGATTTTCTCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCcg                               <  1:151015/133‑1 (MQ=255)                                                                                               aTGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTGACTTCGTCATTATTGTTACTTGGTTCCcgc                              >  1:223127/1‑133 (MQ=255)                                                                                               aTGGCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGATGCCATGTTCCGCTTACTTCGTCCTTATTTTTACTTTGTTCCcgc                              >  2:321136/1‑133 (MQ=255)                                                                                                 ggCATTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCgcgc                            >  2:33716/1‑133 (MQ=255)                                                                                                    aTTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCcgcg                             >  1:368615/1‑129 (MQ=255)                                                                                                    aTTGGGGATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCcgcg                             <  2:368615/129‑1 (MQ=255)                                                                                                          gATTGAATTTATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaa                   <  2:72213/133‑1 (MQ=255)                                                                                                                 tttATCGTTACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaa            <  1:421789/133‑1 (MQ=255)                                                                                                                        ttACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa     <  1:321893/133‑1 (MQ=255)                                                                                                                         tACTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTTCCGCGCACTTAGCATAAGCAATAGGAATAg    >  1:242974/1‑133 (MQ=255)                                                                                                                          aCTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAgg   <  1:33716/133‑1 (MQ=255)                                                                                                                          aCTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAgg   <  1:350529/133‑1 (MQ=255)                                                                                                                          aCTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAgg   <  1:367203/133‑1 (MQ=255)                                                                                                                           cTGCTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGt  <  1:321136/133‑1 (MQ=255)                                                                                                                              |                                                                                                                                  TTGTTGGGGATTGTTCCGCATGGCACCGTGGCTGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGT  >  NC_000913/1299086‑1299340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑