| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,302,986 | G→A | A30T (GCG→ACG) | oppB → | murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,302,986 | 0 | G | A | 96.7% | 92.3 / NA | 30 | A30T (GCG→ACG) | oppB | murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/15); minor base C (0/1); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCA > NC_000913/1302862‑1303113 | aTCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGa < 2:964/133‑1 (MQ=255) acaGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGa > 2:37575/1‑133 (MQ=255) aGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAAc > 2:41943/1‑133 (MQ=255) ccAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTa > 2:340009/1‑133 (MQ=255) aGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCACACGCCGGTAAGTCCTTTTACCGGCGAACGAACTTTACCGc > 2:161640/1‑133 (MQ=255) ggCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAGCGTACTGTACCGCCa > 1:218414/1‑133 (MQ=255) gTTAAAATTTCTTCTTCGTCGCTGCCTGTAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTg < 2:145653/133‑1 (MQ=255) gTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTg < 2:63582/133‑1 (MQ=255) gTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTg > 1:436492/1‑133 (MQ=255) aTTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGTGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTGCCGCCAGAAGTg < 2:31779/127‑1 (MQ=255) aTTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGTGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTg > 1:31779/1‑127 (MQ=255) cTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCg < 1:37575/133‑1 (MQ=255) tGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATatca < 2:290815/133‑1 (MQ=255) gAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATatcatc > 2:98750/1‑133 (MQ=255) gCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTa < 1:326878/133‑1 (MQ=255) gATTCCGAGGCTATTGATCCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAAt < 1:161640/133‑1 (MQ=255) aTTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATg > 2:510365/1‑133 (MQ=255) tCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCTAAATATCATGTTAATGAt > 1:221711/1‑133 (MQ=255) cGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGAt < 1:340009/133‑1 (MQ=255) tttattCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGa < 2:312881/133‑1 (MQ=255) tattCTTATTACTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGaca < 1:510365/133‑1 (MQ=255) attCTTATGACTATTCCGATCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGacac < 1:189107/133‑1 (MQ=255) ttaCTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGaca < 2:335296/125‑1 (MQ=255) ttaCTATTTCGTTCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGaca > 1:335296/1‑125 (MQ=255) cTATTTCGTTCTTTATGATGCGCCCCACTCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAg < 2:246562/133‑1 (MQ=255) aTTTCGTTCTTTATGTTGCGCCTCCCGCTGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCt < 2:146286/133‑1 (MQ=255) ttCTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGa > 1:360895/1‑133 (MQ=255) cTTTATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGaaa < 2:388058/133‑1 (MQ=255) tttATGATGCGCCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAaac > 2:266345/1‑133 (MQ=255) gcCTCACGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCa > 1:291817/1‑133 (MQ=255) | ATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCA > NC_000913/1302862‑1303113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |