Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,310,097	T→A	D58V (GAT→GTT)	kch ←	K(+) channel Kch

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,310,097	0	T	A	100.0%	93.2 / NA	28	D58V (GAT→GTT)	kch	K(+) channel Kch
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/14);  total (14/14)

CGCTATCAACAACAAAATGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGATCCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGT  >  NC_000913/1309973‑1310221                                                                                                                             |                                                                                                                             cgcTATCAACAACAAAATGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCTTAAAGAGTGGGGAGTTGGCCAGCAATGAGACAACCAActct                                                                                                                      >  2:149847/1‑133 (MQ=255) cgcTATCAACAACAAAATGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAActct                                                                                                                      >  1:482708/1‑133 (MQ=255) cgcTATCAACAACAAAATGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAActct                                                                                                                      >  2:302562/1‑133 (MQ=255)              aaaaTGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGa                                                                                                         >  1:307111/1‑133 (MQ=255)               aaaTGATACTGATTGGCCATGCGAGCTTTGCTCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGat                                                                                                        <  1:225879/133‑1 (MQ=255)                          aTTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAatat                                                                                             >  1:402991/1‑133 (MQ=255)                          aTTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAAACAAGTCTGAAAATGCTGTGATATGGAAAatat                                                                                             >  2:144126/1‑133 (MQ=255)                              cccATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTGGAAATGGAAAATATCAAg                                                                                         >  1:201451/1‑133 (MQ=255)                              cccATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTATGAAAATCCTTAGACATGGAAGATATCAAg                                                                                         >  1:212119/1‑133 (MQ=255)                                   gCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAAt                                                                                    >  2:431400/1‑133 (MQ=255)                                       gCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGAc                                                                                <  1:1346/133‑1 (MQ=255)                                       gCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGAc                                                                                <  2:44715/133‑1 (MQ=255)                                       gCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGAc                                                                                <  2:402991/133‑1 (MQ=255)                                       gCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGAc                                                                                <  1:431400/133‑1 (MQ=255)                                       gCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGAc                                                                                <  2:313935/133‑1 (MQ=255)                                           tGCCCGGACCAGTAAACCAATGGAGTTCAGCACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGAc                                                                            <  1:149847/133‑1 (MQ=255)                                           tGCCCGGAACAGTAAACCAATGGAGTTCGGGAAAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGAc                                                                            <  2:212119/133‑1 (MQ=255)                                                     aGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGa                                                                  <  1:315213/133‑1 (MQ=255)                                                           ccAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGttt                                                            <  2:446646/133‑1 (MQ=255)                                                            cAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCGAGCGAATGGACCGACAGACCAGAGATTGtttt                                                           >  2:137801/1‑133 (MQ=255)                                                               tcgAGTTCAAGACAATAAAGACGTGGAGCATATAGAGTGGGCCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTaaa                                                        <  2:242767/131‑1 (MQ=255)                                                                   gTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCGTAGATTGTTATAAAAATa                                                    >  1:242767/1‑133 (MQ=255)                                                                    ttCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATaa                                                   >  1:64187/1‑133 (MQ=255)                                                                       aGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGTTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCa                                                <  1:302562/133‑1 (MQ=255)                                                                                             tAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAg                          <  2:307111/133‑1 (MQ=255)                                                                                                    tGGGGCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCgaga                   >  2:461010/1‑133 (MQ=255)                                                                                                     ggggCGTTTGCCAGCAGGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTGAAAAATAATCAATCCACTTAAAAAGGCGGCGAGCGCGAGaa                  >  2:190700/1‑133 (MQ=255)                                                                                                       ggCGTTTGCCAGCAAGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATg                <  2:64187/133‑1 (MQ=255)                                                                                                                     aGGAGAGAACCAACTCTGAAAATGCTTTGATATGGAAAATATCAACCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGt  <  2:322105/133‑1 (MQ=255)                                                                                                                             |                                                                                                                             CGCTATCAACAACAAAATGATACTGATTGCCCATGCGAGCTTTGCCCGGAACAGTAAGCCAATGGAGTTCAGGACAAGAAAGACGCCGAGCATAAAGAGTGGGGCGTTTGCCAGCAAGGAGAGATCCAACTCTGAAAATGCTTTGATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGT  >  NC_000913/1309973‑1310221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑