| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,311,303 | A→G | E72G (GAA→GGA) | tonB → | Ton complex subunit TonB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,311,303 | 0 | A | G | 84.6% | 29.5 / ‑5.8 | 13 | E72G (GAA→GGA) | tonB | Ton complex subunit TonB |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (10/1); total (12/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCA > NC_000913/1311180‑1311431 | cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCTCAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAcc > 2:314942/1‑133 (MQ=255) ggTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGGGGTAGAACCAGGACCGGAACCt > 1:231322/1‑133 (MQ=255) tACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAGCTGGGCGGATCCCAGAACCGcc > 1:142984/1‑133 (MQ=255) ctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCGGTCCGCGGACCGCCCAAAGAAGc > 1:202774/1‑133 (MQ=255) cgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCAcc > 2:358953/1‑133 (MQ=255) cgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGCAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCAcc > 2:46877/1‑133 (MQ=255) tCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCTATCCCCGAACCGACAAAAGAAGCACTGGTGCTCATTGaaaa > 2:180853/1‑133 (MQ=255) gCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAg > 2:356594/1‑133 (MQ=255) cacaAGCCGTCCAGCAGCCACCGGTGCCAGTGTTAGAGCCACGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGcc < 2:142984/133‑1 (MQ=255) gCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGGAAAGCCGAAGCCGAAATCTAAGCCAAAACCTAAGCCGGTGa > 2:171408/1‑133 (MQ=255) gTTCAGCCGTAACCGGAGCCGGTGGTTGCGCCTGGACCGGATCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaa < 2:231243/133‑1 (MQ=255) ttCAGCCGCCCCCGGCGCCGGTGGTAGAGCCAAGACCGGAAGCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa < 2:202774/133‑1 (MQ=255) ttCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAACCCGAAGCCAAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa > 2:182736/1‑133 (MQ=255) ggtggtAGAGCCAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCaaaa > 2:438796/1‑133 (MQ=255) ccAGGACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCa > 1:37111/1‑133 (MQ=255) | CGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCA > NC_000913/1311180‑1311431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |