Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	85,841	T→C	V71A (GTG→GCG)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	85,841	0	T	C	100.0%	132.5 / NA	39	V71A (GTG→GCG)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

TTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTT  >  NC_000913/85721‑85968                                                                                                                         |                                                                                                                                ttGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTc                                                                                                                     <  2:467941/133‑1 (MQ=255) ttGATATTTAAGATGCATTGCATATCGTTGGTAGTATTGATTATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTc                                                                                                                     <  2:186255/133‑1 (MQ=255)      atTTATGATGAATTGTATACCGTGAGTGCTATTGATCATGTCTTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTc                                                                                                                <  2:136947/133‑1 (MQ=255)        ttATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCa                                                                                                              >  2:289786/1‑133 (MQ=255)          atgatgCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCTACCGGGGAAGTCAGCGGCGCGCTGGTAACGACGGGGCCAgg                                                                                                            >  2:151429/1‑133 (MQ=255)          atgatgCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAGGTCGGCGACGCGCTGGGAACGTCGGGTCCAgg                                                                                                            >  2:134177/1‑133 (MQ=255)          atgatgCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAgg                                                                                                            >  2:26855/1‑133 (MQ=255)                  ttgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGAGAAGTCGGCGTCGCGCTGGTCACGTCGTGTCCAGGGGCGACCa                                                                                                    >  1:231562/1‑133 (MQ=255)                   tgcatACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGGAACGTCGGGTCCCGGGGGGACCaa                                                                                                   >  2:497932/1‑133 (MQ=255)                          cGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGAtt                                                                                            >  1:294971/1‑133 (MQ=255)                            tgggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTAc                                                                                          >  2:340520/1‑133 (MQ=255)                             gggtggTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACt                                                                                         >  2:513928/1‑133 (MQ=255)                             gggtggTATTAATCACGTATTAGTTCGTCATGTGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACt                                                                                         <  2:207129/133‑1 (MQ=255)                              ggtggtATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGACGGGTCCTGGGGCGGCCAATGAGATTACTg                                                                                        >  1:207129/1‑133 (MQ=255)                                   tATTGATCATTTATTTGTTCGTCAAGAGCAGGGGGCGGTGCGTATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGAGATTACTGACATc                                                                                   <  2:193749/133‑1 (MQ=255)                                        aTCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCAc                                                                              >  2:387087/1‑133 (MQ=255)                                        aTCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCAc                                                                              >  1:393356/1‑133 (MQ=255)                                        aTCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCAc                                                                              <  1:513928/133‑1 (MQ=255)                                        aTCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCAc                                                                              >  1:314060/1‑133 (MQ=255)                                           aTGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGc                                                                           <  1:289786/133‑1 (MQ=255)                                            tGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCt                                                                          <  1:497932/133‑1 (MQ=255)                                            tGTATTAGATCGTCACGATCAGCCGGTGGTTCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCt                                                                          <  1:239526/133‑1 (MQ=255)                                             gTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCtt                                                                         >  1:357814/1‑133 (MQ=255)                                                 tAGTTCGTTATGAGCTGGTGGCGGTGCATAAGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTatat                                                                     <  1:154417/133‑1 (MQ=255)                                                 tAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTatat                                                                     <  1:124080/133‑1 (MQ=255)                                                  aGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATg                                                                    >  2:483759/1‑133 (MQ=255)                                                  aGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATg                                                                    >  2:335176/1‑133 (MQ=255)                                                    ttCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGa                                                                  <  1:387087/133‑1 (MQ=255)                                                    ttCGTCATCAGCAGGCGGCGGTTCATATGGACGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGa                                                                  <  1:151429/133‑1 (MQ=255)                                                    ttCGTCATCAGCAGGAGGTGGTACATATGGGCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGa                                                                  <  1:134177/133‑1 (MQ=255)                                                      cGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGGATCTCCACCGCTTATATGGatt                                                                >  1:177738/1‑133 (MQ=255)                                                      cGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGAGACCAATGGGATAACTAGCATCGCCGCCGCCTATATGGatt                                                                >  2:156751/1‑133 (MQ=255)                                                       gTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattc                                                               >  2:407607/1‑133 (MQ=255)                                                       gTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattc                                                               >  2:338413/1‑133 (MQ=255)                                                          aTGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattccat                                                            <  2:31351/133‑1 (MQ=255)                                                             aGCAGGCGGCGGTGCGTATGGCCGTTGGCCTGACGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGattccattcc                                                         <  2:177738/133‑1 (MQ=255)                                                                                     aTGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCATATATGGAGTCCATGCCATTAGTTGGCCTTTACGGGCAGGt                                 >  2:236010/1‑133 (MQ=255)                                                                                            ggCGCGCGCGACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGAcc                          <  1:340520/133‑1 (MQ=255)                                                                                                  cgcgACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCCTTCCATTAATTGGCCTTTCCGGGCAGGTAGCGACCTCGTTg                    >  2:224241/1‑133 (MQ=255)                                                                                                  cgcgACCGGGGAAGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTg                    <  2:294971/133‑1 (MQ=255)                                                                                                             aaGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGa         <  1:335176/133‑1 (MQ=255)                                                                                                             aaGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGa         <  1:483759/133‑1 (MQ=255)                                                                                                             aaGTCGGCGTCGCGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGa         <  1:338413/133‑1 (MQ=255)                                                                                                                    cgtcgcGCTGGTAACGGCGGGTCCAGGGGGGGCCAAAGCGGTTTCTGGGATCGCCACCGCTTATATGGGTTCCCTTTCATTAGTTGTCCTTTCCGGGGAGGTAGCGAACTCGTTGGTAGGGTACGATGGCttt  >  2:357476/1‑133 (MQ=255)                                                                                                                         |                                                                                                                                TTGATATTTATGATGCATTGCATACCGTGGGTGGTATTGATCATGTATTAGTTCGTCATGAGCAGGCGGCGGTGCATATGGCCGATGGCCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACCAATGCGATTACTGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTT  >  NC_000913/85721‑85968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑