Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,620	C→T	N133N (AAC→AAT)	ycjO →	putative ABC transporter membrane subunit YcjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,620	0	C	T	100.0%	87.2 / NA	26	N133N (AAC→AAT)	ycjO	putative ABC transporter membrane subunit YcjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

GGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAACGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTT  >  NC_000913/1373493‑1373745                                                                                                                                |                                                                                                                              ggCTGGCGCTGGCGATGTTTTTGAACCGCGTGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTa                                                                                                                          <  1:205700/133‑1 (MQ=255)  gCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTAc                                                                                                                         >  2:331968/1‑133 (MQ=255)      gCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCa                                                                                                                     <  2:119063/133‑1 (MQ=255)      gCGGTGGCGATGTTTTTCAACCGCAAGTTCCGACTGCGCAATACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCa                                                                                                                     <  2:190111/133‑1 (MQ=255)       cGGTGGCGATGTTTTTCACCCGCGAGTTCCCCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCAt                                                                                                                    <  1:134377/133‑1 (MQ=255)       cGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCATGGAAATACATGTTCAACAATGGCTACGGCAt                                                                                                                    <  1:430076/133‑1 (MQ=255)        ggTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCAtt                                                                                                                   <  2:474573/133‑1 (MQ=255)                             cgAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGAt                                                                                              >  2:378092/1‑133 (MQ=255)                                   ccGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTg                                                                                        <  2:467974/133‑1 (MQ=255)                                          cgcAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATctct                                                                                 >  2:13821/1‑133 (MQ=255)                                            cAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTAt                                                                               <  1:378092/133‑1 (MQ=255)                                                aCCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGc                                                                           <  1:331968/133‑1 (MQ=255)                                                         tCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGc                                                                  <  2:270464/133‑1 (MQ=255)                                                                     ctctCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATa                                                      >  2:86468/1‑133 (MQ=255)                                                                       ctcCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAAt                                                    >  2:81920/1‑133 (MQ=255)                                                                        tcCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATc                                                   <  1:81920/133‑1 (MQ=255)                                                                         ccTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATcc                                                  >  2:120063/1‑133 (MQ=255)                                                                            aCGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCggg                                               <  2:284196/133‑1 (MQ=255)                                                                            aCGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCggg                                               >  1:96868/1‑133 (MQ=255)                                                                                    ccGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCt                                       <  1:38011/133‑1 (MQ=255)                                                                                     cGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCtt                                      <  2:462462/133‑1 (MQ=255)                                                                                             tCGCTGGTGTTCGCCTGGAAATACATGTTCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGATCGATAATCCGGGCAGTAGCTTTGTGCtgg                              <  2:275000/133‑1 (MQ=255)                                                                                                                        ttCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACt   <  1:86468/133‑1 (MQ=255)                                                                                                                        ttCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGATGGTGGTACTGTTCTCCATCTAGCGCTACt   >  1:179063/1‑133 (MQ=255)                                                                                                                        ttCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAACTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACt   >  2:72183/1‑133 (MQ=255)                                                                                                                         tCAACAATGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATAAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACtt  >  2:105170/1‑133 (MQ=255)                                                                                                                                |                                                                                                                              GGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCTACGTAACGCCGTCCATTTCGCTGGTGTTCGCCTGGAAATACATGTTCAACAACGGCTACGGCATTGTTAACTACCTCGGCGTCGATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTT  >  NC_000913/1373493‑1373745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑