Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,376,074	A→G	N14S (AAC→AGC)	ycjR →	putative ketohexose‑3‑epimerase YcjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,074	0	A	G	100.0%	76.9 / NA	25	N14S (AAC→AGC)	ycjR	putative ketohexose‑3‑epimerase YcjR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/17);  total (8/17)

TGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTT  >  NC_000913/1375960‑1376190                                                                                                                   |                                                                                                                     tGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCg                                                                                                    <  1:412373/133‑1 (MQ=255) tGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGAGTCACGTTTTAATTCAAAGGACTGAAAAAAATGAAAATCGGCACCCAGAATCAGGCGGTCTTTCCGGAAAGCATTCTGGAGAAATTTCg                                                                                                    <  2:271225/133‑1 (MQ=255)   cAGTATGTGGACCAGTATACGGGACAGAGCGTCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGtt                                                                                                  <  1:231837/133‑1 (MQ=255)   cAGTATGTCTACCAGCATCCGGAATATACCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTGCGGAAAGCATTCTGGAGAAATTTCGtt                                                                                                  <  2:227424/133‑1 (MQ=255)   cAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGtt                                                                                                  <  1:100701/133‑1 (MQ=255)    aGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTta                                                                                                 <  1:434912/133‑1 (MQ=255)      tATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAGAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTtata                                                                                               >  2:474334/1‑133 (MQ=255)      tATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCTGGCGTTCTATCCGGAAAGCATTCTGTAGAAATTTCGTtata                                                                                               >  2:176002/1‑133 (MQ=255)         gTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCa                                                                                            <  1:467224/133‑1 (MQ=255)         gTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGAGTTCTTTCTGGATAGCATTCTGGAGAAATTGGGTTATATCa                                                                                            >  2:240471/1‑133 (MQ=255)           cGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCaaa                                                                                          >  2:301655/1‑133 (MQ=255)           cGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCaaa                                                                                          >  2:277339/1‑133 (MQ=255)                         aaCAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGa                                                                            <  1:95555/133‑1 (MQ=255)                         aaCAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGa                                                                            <  1:473335/133‑1 (MQ=255)                              agCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGtt                                                                       >  1:361848/1‑133 (MQ=255)                                          ggCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCATTATATCACAGAGATGAGCTTCGATCGTTTAGAGATTGACg                                                           >  2:211672/1‑133 (MQ=255)                                             gTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCa                                                        <  2:351695/133‑1 (MQ=255)                                                 cGTTTTAATTAAAAGGAATGAAAAGAATAAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAAct                                                    <  2:210216/133‑1 (MQ=255)                                                  gTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAActg                                                   <  1:277339/133‑1 (MQ=255)                                                   ttttAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAActgc                                                  <  2:110584/133‑1 (MQ=255)                                                   ttttAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAActgc                                                  <  1:441642/133‑1 (MQ=255)                                                          tCAAAGGACTGAAAAGAAAGAACATCGGCATACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTaa                                           <  1:211672/133‑1 (MQ=255)                                                                   tGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCga                                  <  1:41716/133‑1 (MQ=255)                                                                           aTGAAAATAGGCACATAGGATCAGGCGTTCCTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCa                          <  1:176002/133‑1 (MQ=255)                                                                                              aTCAGGCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAAc       <  2:361848/133‑1 (MQ=255)                                                                                                   gCGTTCTTTCCGGAAAGCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGtt  >  1:9435/1‑133 (MQ=255)                                                                                                                   |                                                                                                                     TGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTT  >  NC_000913/1375960‑1376190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑