Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,144	A→G	T248A (ACC→GCC)	ftsW →	putative lipid II flippase FtsW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,144	0	A	G	100.0%	55.9 / NA	17	T248A (ACC→GCC)	ftsW	putative lipid II flippase FtsW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/8);  total (9/8)

TTTGTGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTA  >  NC_000913/99015‑99271                                                                                                                                  |                                                                                                                                tttgtGACTACGCTGACGATGTCGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCg                                                                                                                              <  1:231093/133‑1 (MQ=255)           cGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGaa                                                                                                                    <  1:293095/133‑1 (MQ=255)               ggCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGGATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCg                                                                                                                >  1:20621/1‑133 (MQ=255)                gCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCTGt                                                                                                               <  2:399908/133‑1 (MQ=255)                gCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGt                                                                                                               <  1:116536/133‑1 (MQ=255)                                                                     tATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGttt                                                          >  1:368392/1‑133 (MQ=255)                                                                             tttCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCgcgg                                                  >  2:49876/1‑133 (MQ=255)                                                                             tttCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCATTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCgcgg                                                  >  2:316753/1‑133 (MQ=255)                                                                               tCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCgcggcg                                                >  2:508256/1‑133 (MQ=255)                                                                               tCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGACTATCAG‑TAACGGAATGGGTGGTGGACGTTGGTTgcgggcg                                               >  2:154718/1‑130 (MQ=255)                                                                                   cGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGTTGGGGTTTGGTCGCGGCGAACt                                            >  1:173118/1‑133 (MQ=255)                                                                                     gTTGTGTTGATGATCCTCGCCGACACGTTCCGCATCCGCCGGGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACttt                                          <  2:232916/133‑1 (MQ=255)                                                                                           ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                    <  2:287697/133‑1 (MQ=255)                                                                                           ttGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                    <  1:49876/133‑1 (MQ=255)                                                                                               tGATACTCGCCGAACCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAgg                                >  1:467129/1‑133 (MQ=255)                                                                                                           aaCCGTACCGTATCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTc                    <  2:368392/133‑1 (MQ=255)                                                                                                                taccgtaTCCGCCGTGTTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTAc               <  1:475503/133‑1 (MQ=255)                                                                                                                             gtgtTGCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTa  >  1:5976/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                TTTGTGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTA  >  NC_000913/99015‑99271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑