Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,438,800	T→C	D662G (GAC→GGC)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,438,800	0	T	C	100.0%	77.7 / NA	24	D662G (GAC→GGC)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/7);  total (17/7)

AGCGGCAACGCAGTGGTTACATTGGGTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGTCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTT  >  NC_000913/1438691‑1438925                                                                                                              |                                                                                                                              aGCGGCAACGCAGTGGTTACATTGGGTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACgggg                                                                                                        <  1:334745/133‑1 (MQ=255)            aGTGGTTACATTGGGTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCAcc                                                                                             >  1:504351/1‑133 (MQ=255)                        gggTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGc                                                                                    <  2:422843/130‑1 (MQ=255)                        gggTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGc                                                                                    >  1:422843/1‑130 (MQ=255)                          gTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCa                                                                               >  1:311699/1‑133 (MQ=255)                           tACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCAt                                                                              >  1:255114/1‑133 (MQ=255)                           tACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCAt                                                                              >  1:293629/1‑133 (MQ=255)                               gagTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCa                                                                          >  2:508632/1‑133 (MQ=255)                               gagTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCa                                                                          <  1:103505/133‑1 (MQ=255)                               gagTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCa                                                                          >  1:86251/1‑133 (MQ=255)                                agTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCCCCAAGCCCGGCGAACATCGCAg                                                                         >  1:199602/1‑133 (MQ=255)                                agTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAg                                                                         >  2:382843/1‑133 (MQ=255)                                     ctctTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGtt                                                                    >  2:331947/1‑133 (MQ=255)                                               aTGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCTCGGCGAGCATCGCAGCGGTTAGGGTTTTCa                                                          >  1:233000/1‑133 (MQ=255)                                                         ctTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAgg                                                >  2:416140/1‑133 (MQ=255)                                                           tCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAgggg                                              <  1:382843/133‑1 (MQ=255)                                                               cGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCTGCGGTTACGGTTTTCACGAAATCAGGggcggc                                          >  1:9627/1‑133 (MQ=255)                                                                 atatTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCAt                                        >  1:58686/1‑133 (MQ=255)                                                                                 aGCGCGTAGTGCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCgg                        <  2:311699/133‑1 (MQ=255)                                                                                          tGCCCATCGGCGAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGTGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGgc               <  2:102295/133‑1 (MQ=255)                                                                                           gCCCATCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGgcg              <  1:492387/133‑1 (MQ=255)                                                                                               aTCGGCCAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACAACTCGCCGTCGATTGGCGCtgt          >  1:191854/1‑133 (MQ=255)                                                                                                    ccAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCtg           >  1:479061/1‑127 (MQ=255)                                                                                                    ccAGGTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCtg           <  2:479061/127‑1 (MQ=255)                                                                                                       ggTGCCGCCTGGCGGCAGCGCCGAAACGGGGAGGGCGGCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCAGTGGGGGTCGATTGGCGCAGTGGGGGttt  >  2:207636/1‑133 (MQ=255)                                                                                                              |                                                                                                                              AGCGGCAACGCAGTGGTTACATTGGGTACAGAGTTCCTCTTTCCAGATGGGGATCTCTTCGGCGATATTGCGTTTTTCCCAGCGCGTAGTGCCCATCGGCCAGGTGCCGTCTGGCGGCAGCGCCGAAACGGGGAGGGCGTCACCAAGCCCGGCGAGCATCGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTT  >  NC_000913/1438691‑1438925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑