Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,478,201 T→C V194A (GTG→GCG)  ynbA → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,478,2010TC100.0% 114.7 / NA 34V194A (GTG→GCG) ynbAputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

TATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGG  >  NC_000913/1478081‑1478328
                                                                                                                        |                                                                                                                               
tatTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAg                                                                                                                     <  1:463505/133‑1 (MQ=255)
tatTTGGTCTCTGGGTTCTGGCCGTTGCTATTTATCCACAGCGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAg                                                                                                                     <  2:153252/133‑1 (MQ=255)
  tttGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAgcg                                                                                                                   <  1:262299/133‑1 (MQ=255)
      gTCTGTGGGGTCTGACCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGGGCGCTTCTTATGAGCGCTGa                                                                                                               >  2:494366/1‑133 (MQ=255)
       tCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGaa                                                                                                              >  2:420178/1‑133 (MQ=255)
       tCTGTGGGGCCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATGGTTGTGGGAGTGCGCTGCTTAGGAGCGCTGaa                                                                                                              >  2:229097/1‑133 (MQ=255)
                 cTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCt                                                                                                    >  1:407869/1‑133 (MQ=255)
                   ggCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCCCTTCTTAGGAGTGCTGAAATAGAATGCTgg                                                                                                  >  1:251402/1‑133 (MQ=255)
                                     acaGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAacac                                                                                >  2:73378/1‑133 (MQ=255)
                                       aGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATCAGCGCTGAAATAGAATGGTGGAAAATTCTCAAGCAacacat                                                                              >  2:244594/1‑131 (MQ=255)
                                         tGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACtttt                                                                            >  1:291467/1‑133 (MQ=255)
                                         tGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACtttt                                                                            >  1:434844/1‑133 (MQ=255)
                                           gATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTcg                                                                          <  2:294918/133‑1 (MQ=255)
                                           gATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTcg                                                                          <  1:73378/133‑1 (MQ=255)
                                            aTGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTcgc                                                                         <  1:299791/133‑1 (MQ=255)
                                             tGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTcgcg                                                                        <  1:420178/133‑1 (MQ=255)
                                                 gTGGAATAAACTTTTATGGAGCACCGCTTCAATTCTGCTTCCCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCgttgt                                                                    <  2:251402/133‑1 (MQ=255)
                                                     aataatCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAAt                                                                >  2:28027/1‑133 (MQ=255)
                                                       taatCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAAttt                                                              >  1:64727/1‑133 (MQ=255)
                                                                           cTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGt                                          >  1:406045/1‑133 (MQ=255)
                                                                           cTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGt                                          >  1:362822/1‑133 (MQ=255)
                                                                            ttCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGtt                                         <  1:28027/133‑1 (MQ=255)
                                                                               aaTTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTc                                      <  2:434844/133‑1 (MQ=255)
                                                                                      cTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTGAGCAACGTTGATAAACCGTTTGCTGCTATg                               >  1:216101/1‑133 (MQ=255)
                                                                                      cTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGCGCTTCTGATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATg                               <  1:396425/133‑1 (MQ=255)
                                                                                                 tGCGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTTTGCTATGGCGGTTACCgg                    <  2:407869/133‑1 (MQ=255)
                                                                                                   cGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGgag                  >  2:328811/1‑133 (MQ=255)
                                                                                                   cGATTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGgag                  <  2:406045/133‑1 (MQ=255)
                                                                                                     aTTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTGTGGCGGTTTCCgggggg                >  2:103102/1‑129 (MQ=255)
                                                                                                     aTTAATCGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGgaggg                >  2:486200/1‑131 (MQ=255)
                                                                                                           cGTTGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAgg          <  2:438506/133‑1 (MQ=255)
                                                                                                             ttGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGGGAGGAAAgggg        >  2:260607/1‑133 (MQ=255)
                                                                                                              tGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAggggg       <  2:291467/133‑1 (MQ=255)
                                                                                                              tGTCGGAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAggggg       <  1:494366/133‑1 (MQ=255)
                                                                                                              tGTCGGAGTGCGCTTATTATGACCGCTGAACTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAggggg       <  1:155430/133‑1 (MQ=255)
                                                                                                                   gAGTGCGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAgg  <  1:328811/133‑1 (MQ=255)
                                                                                                                        |                                                                                                                               
TATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGG  >  NC_000913/1478081‑1478328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑