Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,238	T→C	S110S (AGT→AGC)	ynbD →	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,238	0	T	C	100.0%	89.7 / NA	27	S110S (AGT→AGC)	ynbD	putative phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

TTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTACTTTGGCGTCACTTTCGTCAGCATCTGGCTGAGAG  >  NC_000913/1481113‑1481363                                                                                                                              |                                                                                                                              ttCGTTTGTAGCACGACATTCGAGCTGCGCCGACTTGTCCGCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGa                                                                                                                        <  2:43921/133‑1 (MQ=255)              cGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTAtt                                                                                                           >  2:16492/1‑133 (MQ=255)                   ttCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAATTGAGCGGGGTGACGTGATGGCTATTTTCGc                                                                                                      >  1:201572/1‑133 (MQ=255)                              ccGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTg                                                                                           >  2:312383/1‑133 (MQ=255)                              ccGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTg                                                                                           >  1:378807/1‑133 (MQ=255)                                    tGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                                                                     <  1:101568/133‑1 (MQ=255)                                    tGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                                                                     <  1:312383/133‑1 (MQ=255)                                     gTCCACCGGCTTATTGTGGCAACGCTTACGGCCTGCTGCGGCTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATc                                                                                    <  2:242109/133‑1 (MQ=255)                                      tCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                                                                     >  1:443047/1‑131 (MQ=255)                                      tCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGAt                                                                                     <  2:443047/131‑1 (MQ=255)                                      tCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCt                                                                                   <  2:345129/133‑1 (MQ=255)                                      tCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATAGCTATTTTCGCAACTTGAACTGTTTGATCt                                                                                   >  1:59580/1‑133 (MQ=255)                                             gCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTtat                                                                            >  1:452746/1‑133 (MQ=255)                                                tATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAAc                                                                         >  2:398515/1‑133 (MQ=255)                                                       gCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGtctc                                                                  >  1:51544/1‑133 (MQ=255)                                                            ggTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCg                                                             <  1:16492/133‑1 (MQ=255)                                                            ggTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCg                                                             <  2:378807/133‑1 (MQ=255)                                                              tAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCt                                                           <  2:395522/133‑1 (MQ=255)                                                                 tGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCg                                                             <  1:11069/128‑1 (MQ=255)                                                                 tGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCg                                                             >  2:11069/1‑128 (MQ=255)                                                                        ctgcGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATattatt                                                 <  1:431540/133‑1 (MQ=255)                                                                                               tGACGTTTAGTTTTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCACCTTCGCTACATGTAATACTCTGCTAGCTAGGTTGGCGtaa                          >  2:245821/1‑131 (MQ=255)                                                                                                    tttagtttTATCCGTCCTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGTTGGCTAATTTGGCGTCACTTTc                     >  1:232953/1‑133 (MQ=255)                                                                                                      tagtttTATCAGTCCGGAAGTGAGCGGGGTGAAGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTACTTTGGCGTCACTTTCGt                   <  2:201572/133‑1 (MQ=255)                                                                                                                   ccTGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTAATTTGGCGACACGTTAGTCAGCGTCTGGCTg      >  1:139120/1‑133 (MQ=255)                                                                                                                     tGAAGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTACTTTGGCGTCACTTTCGTCAGCATCTGGCTgag    <  1:398515/133‑1 (MQ=255)                                                                                                                       aaGTGAGCGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTACTTTGGCGTCACTTTCGTCAGCATCTGGCTGagag  <  2:452876/133‑1 (MQ=255)                                                                                                                              |                                                                                                                              TTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAACTGTTTGATCTGCCTTATAACCAGTCTCCTTCGCTGCATATTATTCTCTGCTGGCTACTTTGGCGTCACTTTCGTCAGCATCTGGCTGAGAG  >  NC_000913/1481113‑1481363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑