| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,533,261 | A→G | K70K (AAA→AAG) | pptA → | putative 4‑oxalocrotonate tautomerase (4‑OT) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,533,261 | 0 | A | G | 100.0% | 50.6 / NA | 17 | K70K (AAA→AAG) | pptA | putative 4‑oxalocrotonate tautomerase (4‑OT) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/1); total (16/1) | |||||||||||
GTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCG > NC_000913/1533133‑1533381 | gTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTg < 1:321994/133‑1 (MQ=255) attattCGTCATCTGAACAGTAAATACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGtt > 1:347533/1‑133 (MQ=255) attattCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGcc > 2:471709/1‑128 (MQ=255) cGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGcc > 2:355652/1‑122 (MQ=255) gTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCAt > 2:95488/1‑133 (MQ=255) tCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCTAAATGGAGTCTTAGATCAAGAAGCCTGGTTATAGCATGAAt > 2:194942/1‑133 (MQ=255) tAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGACTTTGATAAAGATGCCAGGTTATAGAATGAATGCTTAAccc > 2:218109/1‑133 (MQ=255) gTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAac > 1:59710/1‑133 (MQ=255) cagcagATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTGACCTGCCGTAACACGCCTGCCGCTGGTAACaa > 2:217423/1‑133 (MQ=255) gATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCCCCGAAACACGCTTGCCCCTGGGAACAAACGTc > 2:188866/1‑133 (MQ=255) ttCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAACATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTccc > 1:65054/1‑133 (MQ=255) aaCCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAAGACGCTTGGCGCTGGCAACAAAAGTCCCAGc > 1:192210/1‑133 (MQ=255) aGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCTGCGCCTAAAGGATGCAAc > 1:129687/1‑133 (MQ=255) tGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCGGCACCTAAATGGTGAAACGTACTGAGCTTa > 1:226521/1‑133 (MQ=255) cccAAATGGAGGCTTTGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATACAACGTAATGAACTAATCATCATCAAACTg > 1:224804/1‑133 (MQ=255) tttGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGGTTATCATAATCTAACTGCCAGCGGCCTTCg > 1:215755/1‑133 (MQ=255) tttGATAAAGAAGCCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCg > 2:306271/1‑133 (MQ=255) | GTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCG > NC_000913/1533133‑1533381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |