| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,542,818 | C→T | G415S (GGC→AGC) | narU ← | nitrate/nitrite transporter NarU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,542,818 | 0 | C | T | 95.5% | 66.0 / NA | 22 | G415S (GGC→AGC) | narU | nitrate/nitrite transporter NarU |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (10/11); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCCGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCGATAAATGGTTATCTGGCGAAAGATGA > NC_000913/1542692‑1542946 | gccgACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCAc > 2:259700/1‑133 (MQ=255) ccgACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCCGCCCACt < 2:302119/133‑1 (MQ=255) aTAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCa < 1:442395/133‑1 (MQ=255) aTAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCa < 1:283239/133‑1 (MQ=255) tAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCaa < 2:48689/133‑1 (MQ=255) ccagGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTgag > 1:123737/1‑133 (MQ=255) agcaCACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCa > 1:264598/1‑133 (MQ=255) tGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGt < 2:123737/133‑1 (MQ=255) aTCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACCGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAg < 2:413674/133‑1 (MQ=255) gCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAATCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCg < 1:119535/133‑1 (MQ=255) aGCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTa > 1:371642/1‑133 (MQ=255) gCGACATGCCAAACGTCTTCGGTATAAAAAGGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTAc < 1:189160/133‑1 (MQ=255) gCGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTAc < 1:259700/133‑1 (MQ=255) cGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTAcc > 1:431914/1‑133 (MQ=255) cGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTAc < 1:62573/132‑1 (MQ=255) cGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTAc > 2:62573/1‑132 (MQ=255) cGACATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTACTTTATGATCTTTCTCTTCACTACCACCTTACATCTTTAcc > 2:189160/1‑133 (MQ=255) aCATGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCg > 1:354828/1‑133 (MQ=255) aTGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCGAt < 2:431914/133‑1 (MQ=255) tGCCAAACGCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCGATa > 1:345767/1‑133 (MQ=255) gCCTGCGGAATAAAAAAGCTGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTTCTCATCACGACCGCCTTTCATCTTTTCCCGGTAAATGGTTa > 2:141812/1‑133 (MQ=255) aaGCTGCCAACTGCGCCAGTGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCGATAAATGGTTATCTGGCGAAAGATGa < 1:141812/133‑1 (MQ=255) | GCCGACCATAAACCAGCCAGGTCAGCAGCACACAAACGATGTAGAAGATTAAAAACACTTTCATCGCGCCGACCGGAGAGCCGGTCATATTGAGCGACATGCCAAACGCCTGCGGAATAAAAAAGCCGCCCACTGCGCCAATGGCTGAGATAAAGCCCAGAGCCGCCGCCGTTTCGGTGACGGCTTCTTTATGAGCTTGCTCATCACTACCGCCTTTCATCTTTACCCGATAAATGGTTATCTGGCGAAAGATGA > NC_000913/1542692‑1542946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |