Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	106,009	T→C	R235R (CGT→CGC)	ftsZ →	cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	106,009	0	T	C	100.0%	106.1 / NA	31	R235R (CGT→CGC)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

TGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGTGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACGGT  >  NC_000913/105882‑106137                                                                                                                                |                                                                                                                                 tgCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGga                                                                                                                             >  1:462101/1‑133 (MQ=255)         aTCGCTGAACAGATTACTCGTCCGCGTTTGATTAACGTGGATTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAgctg                                                                                                                     <  2:210590/133‑1 (MQ=255)           cGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAgctgct                                                                                                                   >  1:85065/1‑133 (MQ=255)                               cGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTtctc                                                                                                <  1:260564/132‑1 (MQ=255)                               cGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTtctc                                                                                                >  2:260564/1‑132 (MQ=255)                                        tgaACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGa                                                                                      <  2:72287/133‑1 (MQ=255)                                                     tGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATATCTTCTCAGCTACTAGAAGATATCGACCTg                                                                         >  1:252888/1‑133 (MQ=255)                                                     tGCAGACGTACGCACCGTAATGTCTGAGATGCGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTg                                                                         >  2:474772/1‑133 (MQ=255)                                                            gTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGgcg                                                                  >  2:29893/1‑133 (MQ=255)                                                             tACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCGGCTGCTGGAAGCTATCGACCTGTCTGgcgc                                                                 >  1:155004/1‑133 (MQ=255)                                                               cGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTAGCTCTTCTCTGCTGCTGAAAGATATCTACCTTTCTGgcgcgc                                                               >  2:157259/1‑133 (MQ=255)                                                                  aCCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGcgcgcgcg                                                            <  1:268265/133‑1 (MQ=255)                                                                      tAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGcgcgcgcg                                                            <  1:6796/129‑1 (MQ=255)                                                                      tAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGcgcgcgcg                                                            >  2:6796/1‑129 (MQ=255)                                                                        aTGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGc                                                      <  1:29893/133‑1 (MQ=255)                                                                                gaTGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAAc                                              >  1:346398/1‑133 (MQ=255)                                                                                gaTGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAAc                                              >  1:270381/1‑133 (MQ=255)                                                                                    ggCTTCGCAATGATGGGTTCTGAGGTGGCTAGCGGTGAACTTAGTGAGGCAGAAGCTGCTGAAATGGCAATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCTGCGTGCTGGTTGACATcg                                          >  2:112743/1‑132 (MQ=255)                                                                                                  gggTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGAc                            <  2:85065/133‑1 (MQ=255)                                                                                                  gcgTTCTGGACTGGCCAGCGGTGCTGCCCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGAc                            <  2:252888/131‑1 (MQ=255)                                                                                                   ggTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGAcc                           >  2:477053/1‑133 (MQ=255)                                                                                                   ggTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGAc                            <  2:260488/132‑1 (MQ=255)                                                                                                   ggTTCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGAc                            >  1:260488/1‑132 (MQ=255)                                                                                                      tCTGGCGTGGCGAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGc                        <  2:1302/133‑1 (MQ=255)                                                                                                        tggcgtggcgAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCgg                      >  1:359887/1‑132 (MQ=255)                                                                                                        tggcgtggcgAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGt                      >  2:278917/1‑133 (MQ=255)                                                                                                        tggcgtggcgAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGt                      >  2:29692/1‑133 (MQ=255)                                                                                                        tggcgtggcgAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAAAATCACGGCGGGCTTCGACCTGCgg                      >  2:219799/1‑132 (MQ=255)                                                                                                              ggcgAGCGGTGAAGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGAt                <  1:278917/133‑1 (MQ=255)                                                                                                                          aGACCGCGCGGAAGACGCTGCTGAAATGTCTAACTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACg    <  2:161564/133‑1 (MQ=255)                                                                                                                          aGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACg    <  1:80624/133‑1 (MQ=255)                                                                                                                          aGACCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACg    <  2:462101/133‑1 (MQ=255)                                                                                                                          aGACCGCGCGCAAGAAGCTGCTGACATGCCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACg    <  2:190673/133‑1 (MQ=255)                                                                                                                            aCCGCGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTACGACCTGCGTCCGGTTGAGTTCGGAAAggt  >  1:228843/1‑133 (MQ=255)                                                                                                                                |                                                                                                                                 TGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGAGATGGGCTACGCAATGATGGGTTCTGGCGTGGCGAGCGGTGAAGACCGTGCGGAAGAAGCTGCTGAAATGGCTATCTCTTCTCCGCTGCTGGAAGATATCGACCTGTCTGGCGCGCGCGGCGTGCTGGTTAACATCACGGCGGGCTTCGACCTGCGTCTGGATGAGTTCGAAACGGT  >  NC_000913/105882‑106137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑