Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,559,226	C→A	A229S (GCC→TCC)	ddpC ←	putative D,D‑dipeptide ABC transporter membrane subunit DdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,559,226	0	C	A	100.0%	98.9 / NA	34	A229S (GCC→TCC)	ddpC	putative D,D‑dipeptide ABC transporter membrane subunit DdpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/14);  total (20/14)

CCCCCGGAAATGCGCAATACCACCACTGATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAG  >  NC_000913/1559106‑1559353                                                                                                                         |                                                                                                                                cccccGGACATGCGCCATTCCATCACTGATTGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATcgc                                                                                                                     <  1:214155/133‑1 (MQ=255)             cgcAATACCACCACTGATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATc                                                                                                         >  2:254876/1‑133 (MQ=255)             cgcAATACCACCACTGATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATc                                                                                                         >  1:382085/1‑133 (MQ=255)                   accaccACTGATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGa                                                                                                   <  1:442233/133‑1 (MQ=255)                            gATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCAc                                                                                          >  2:339774/1‑133 (MQ=255)                             aTCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGAACATTAAATTCGAGCTCCCGATATCCAGCGATGCCTGCACg                                                                                         >  2:182853/1‑133 (MQ=255)                               cGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGAt                                                                                       >  2:379084/1‑133 (MQ=255)                                  gTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAg                                                                                    <  1:426577/133‑1 (MQ=255)                                   tACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAgc                                                                                   <  1:379084/133‑1 (MQ=255)                                       tAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCCCGATCAgcggcg                                                                               >  1:66441/1‑133 (MQ=255)                                       tAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAgcggcg                                                                               >  1:447768/1‑133 (MQ=255)                                          ttGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTa                                                                            <  2:382085/133‑1 (MQ=255)                                            gCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCTAGCGATGTCTGAACGATCAGCGGCGGTAgg                                                                          >  1:143649/1‑133 (MQ=255)                                            gCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGGAgg                                                                          >  2:29908/1‑133 (MQ=255)                                             cGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGGAggg                                                                         >  1:302213/1‑133 (MQ=255)                                               cccATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAg                                                                       <  1:454582/132‑1 (MQ=255)                                               aCCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAg                                                                       <  2:403984/133‑1 (MQ=255)                                                     tGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGt                                                                 <  1:492804/133‑1 (MQ=255)                                                             tCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATg                                                         >  2:122997/1‑133 (MQ=255)                                                              cgcgCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGc                                                        >  2:509341/1‑133 (MQ=255)                                                                cgcCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCa                                                      >  2:446430/1‑133 (MQ=255)                                                                 gcCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAg                                                     >  2:49537/1‑133 (MQ=255)                                                                       ttCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGCAGGGAGTAACGTAAAATATGCGAGTTGATc                                               >  1:178070/1‑133 (MQ=255)                                                                               tCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGGGGTAGGGAATTACGCATAATATGCCAGTTGGTCAGATGCCa                                       >  2:223554/1‑133 (MQ=255)                                                                                            aCCCAGGCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCa                          <  1:254876/133‑1 (MQ=255)                                                                                                 ggCCAATAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGt                     >  1:472917/1‑133 (MQ=255)                                                                                                       tAAATCCCAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTcgc               >  1:312120/1‑133 (MQ=255)                                                                                                            cccAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCGAACGAGAGGCGGCAAAGGTTTACGCCGCCt          >  1:139626/1‑133 (MQ=255)                                                                                                             ccAACGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTg         <  2:302213/133‑1 (MQ=255)                                                                                                               aaCGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGaa       <  1:98346/133‑1 (MQ=255)                                                                                                               aaCGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGaa       <  2:464469/133‑1 (MQ=255)                                                                                                               aaCGTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGaa       >  2:64985/1‑133 (MQ=255)                                                                                                                aCGTGGCGGAAATTAAAATCGCGCTACCTATAACCAGCGATTCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAAc      <  2:183352/133‑1 (MQ=255)                                                                                                                  gTGGCGGACATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACAt    <  1:472431/133‑1 (MQ=255)                                                                                                                    ggcggaCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAg  <  1:122997/133‑1 (MQ=255)                                                                                                                     gcggaCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTcgccgc            <  2:16232/122‑1 (MQ=255)                                                                                                                     gcggaCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTcgccgc            >  1:16232/1‑122 (MQ=255)                                                                                                                         |                                                                                                                                CCCCCGGAAATGCGCAATACCACCACTGATCGAGTACATAGTTGCGACCATTTGCTACCATCGCGCCCCATTCAGCACTCGGTTGTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAG  >  NC_000913/1559106‑1559353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑