| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,593,020 | G→A | pseudogene (5067/5422 nt) | yneO ← | AIDA‑I family autotransporter YneO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,593,020 | 0 | G | A | 92.6% | 78.8 / NA | 28 | pseudogene (5067/5422 nt) | yneO | AIDA‑I family autotransporter YneO |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (9/16); minor base C (0/2); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGA > NC_000913/1592893‑1593135 | gAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTTGAAATCt > 2:93976/1‑133 (MQ=255) aCTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCttt < 2:311763/133‑1 (MQ=255) tttACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATc > 1:49659/1‑133 (MQ=255) tttACCATCGTCACGATGGTGATGGCTGGTCAGGTAGGTTTACACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATc < 2:189255/133‑1 (MQ=255) ccATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAgg < 1:447403/133‑1 (MQ=255) aCGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTgc > 1:469732/1‑133 (MQ=255) gCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTgcgc > 2:455603/1‑133 (MQ=255) gTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTACGGTCGAAAACTTTCACACCCATCCAGGTGAGTTGCGCCtgt > 2:163082/1‑133 (MQ=255) gATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTgg < 2:109109/133‑1 (MQ=255) gATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTgg < 2:126685/133‑1 (MQ=255) ttCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGt > 1:45258/1‑133 (MQ=255) ttCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTAATTGGCGCCTGTGACTGGACGt > 1:153055/1‑133 (MQ=255) aggtaggtTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTAcc < 1:311111/133‑1 (MQ=255) gtaggtTTTCACCCCAAGTCGCGTTTGTACATTTCCGTCGCCTTCCGTTTCAATGCCCGTTCCGTCTTTCCGGGTATGGCCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAg > 2:300770/1‑133 (MQ=255) ggtTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTa < 1:455603/133‑1 (MQ=255) cACCCCAACTCGCGTTTGGACACTTCCGTCCCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGc < 1:173589/133‑1 (MQ=255) aCCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCg > 1:35729/1‑133 (MQ=255) aaGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCt > 1:102448/1‑133 (MQ=255) gcgTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCt < 2:469732/133‑1 (MQ=255) cGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTcc < 1:76860/133‑1 (MQ=255) cGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTcc < 1:300770/133‑1 (MQ=255) cTTCCGTTTCAATGCCCGTTCCGTTTTTCCGGGTATGGTCGCAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTc < 2:134621/133‑1 (MQ=255) cTTCCGTTTCAATGCCCGTTCCGTCTCTCCGGGTATGGTCGCAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTc < 2:223058/133‑1 (MQ=255) ttCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCa < 1:96256/133‑1 (MQ=255) ttCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCa < 2:49659/133‑1 (MQ=255) gTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAgg < 1:93976/133‑1 (MQ=255) aTGCGCGTCCCGTCTTTCGGGGAATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATacc < 1:173159/133‑1 (MQ=255) tGCGCGTTCCGTCTTTCCGGGTATGGTCGAAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATaccc < 1:14261/133‑1 (MQ=255) cTTTCCGGTTATGGTGGACATCATTCACACACATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATACCCACCCTCAACaga < 1:163082/133‑1 (MQ=255) | GAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATACCCACCCTCAACAGA > NC_000913/1592893‑1593135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |