Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,766	A→G	T584A (ACC→GCC)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,766	0	A	G	100.0%	128.9 / NA	38	T584A (ACC→GCC)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

CCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTG  >  NC_000913/124636‑124880                                                                                                                                   |                                                                                                                   cccGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGcc                                                                                                                  >  2:90114/1‑133 (MQ=255) cccGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGcc                                                                                                                  >  1:93206/1‑133 (MQ=255)         gTCAGCAGTAAACCCTGCAGGACCGCTATCAGTTTGCTTACAATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAg                                                                                                          <  2:235140/133‑1 (MQ=255)               agTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACcaa                                                                                                    <  1:477569/133‑1 (MQ=255)                        cGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGcc                                                                                           >  1:408360/1‑133 (MQ=255)                           aGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCgat                                                                                        >  2:96867/1‑133 (MQ=255)                           aGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCgat                                                                                        >  1:354464/1‑133 (MQ=255)                               ccGCGAGCTGGTTGCTTATTATTAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATc                                                                                    <  1:247705/133‑1 (MQ=255)                                  cgAGCAGGTTGCTTACTATAAAGACGACTAGACAGGTCAGACTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCg                                                                                 <  2:144045/133‑1 (MQ=255)                                    aGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGtt                                                                               >  2:405357/1‑133 (MQ=255)                                    aGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGtt                                                                               <  2:305914/133‑1 (MQ=255)                                       aGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTa                                                                            >  2:69079/1‑133 (MQ=255)                                        ggTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTAc                                                                           <  1:75578/133‑1 (MQ=255)                                            gCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCt                                                                       <  2:93206/133‑1 (MQ=255)                                             cTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTa                                                                      >  2:327807/1‑133 (MQ=255)                                                   ataAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGCTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTc                                                                >  2:306597/1‑133 (MQ=255)                                                       agaagaCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATg                                                            >  1:121988/1‑133 (MQ=255)                                                        gaagaCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAGCAATCTGCGGATTATCTCGTTCTACCTCTAGTACTCGATGt                                                           >  2:142734/1‑133 (MQ=255)                                                           gaCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCg                                                        <  2:121988/133‑1 (MQ=255)                                                            aCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCgg                                                       >  1:115329/1‑133 (MQ=255)                                                            aCGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAAACTGCCGATGATCCCGTGCTACATCTATTACACGATGTTCgg                                                       >  2:194910/1‑133 (MQ=255)                                                                gaAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTc                                                   <  2:115329/133‑1 (MQ=255)                                                                   aGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAg                                                >  2:435109/1‑133 (MQ=255)                                                                     gTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCg                                              >  2:317882/1‑133 (MQ=255)                                                                       cAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTa                                            <  2:40631/133‑1 (MQ=255)                                                                          aTTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTg                                         <  1:96867/133‑1 (MQ=255)                                                                              tGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGa                                     >  2:439360/1‑133 (MQ=255)                                                                               gCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGAt                                    <  1:439360/133‑1 (MQ=255)                                                                               gCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGCTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGAt                                    <  1:306597/133‑1 (MQ=255)                                                                                cAGGAAGGTATCAACGAGATGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATc                                   <  1:215658/133‑1 (MQ=255)                                                                                   gAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCtgt                                >  1:57890/1‑133 (MQ=255)                                                                                          tCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggc                         >  1:454337/1‑133 (MQ=255)                                                                                            aaCGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGggcgg                       <  2:454337/133‑1 (MQ=255)                                                                                                  cTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCg                 <  2:354464/133‑1 (MQ=255)                                                                                                   tGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGa                >  2:398830/1‑133 (MQ=255)                                                                                                            gttgttCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAgc       <  1:78080/133‑1 (MQ=255)                                                                                                               gttCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGcgcg    <  2:408360/133‑1 (MQ=255)                                                                                                                 tCCTGGCTGGCAGCGGCGGCCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTg  <  1:405357/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                   CCCGAACGGTCAGCAGTACACCCCGCAGGACCGCGAGCAGGTTGCTTACTATAAAGAAGACGAGAAAGGTCAGATTCTGCAGGAAGGGATCAACGAGCTGGGCGCAGGTTGTTCCTGGCTGGCAGCGGCGACCTCTTACAGCACCAACAATCTGCCGATGATCCCGTTCTACATCTATTACTCGATGTTCGGCTTCCAGCGTATTGGCGATCTGTGCTGGGCGGCTGGCGACCAGCAAGCGCGTG  >  NC_000913/124636‑124880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑