| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,608,512 | G→A | R171C (CGC→TGC) | yneE ← | conserved inner membrane protein YneE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,608,512 | 0 | G | A | 95.7% | 68.6 / NA | 24 | R171C (CGC→TGC) | yneE | conserved inner membrane protein YneE |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/11); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAATCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCG > NC_000913/1608382‑1608638 | aaaaTCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACCGGATATCCGAAAGCGGTCCATCGCGGCACt > 1:176543/1‑133 (MQ=255) aTCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGaa < 2:65151/133‑1 (MQ=255) gtgtAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCa > 1:264332/1‑133 (MQ=255) tAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAAcc > 1:455859/1‑133 (MQ=255) aGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGATAGCGGTCTATTGCGGCGCTGAACCGCCAACCa > 2:145522/1‑133 (MQ=255) aaGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATtctc > 1:119359/1‑133 (MQ=255) gCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTaa < 2:455859/133‑1 (MQ=255) gCGTATAGCCAATGCCCTCGCACCCTGCCATGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTaa < 1:145522/133‑1 (MQ=255) taGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGa > 2:471942/1‑133 (MQ=255) ggCAATGCTCTCGCACCCTGCCCGGACCGCAGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATa < 1:142896/133‑1 (MQ=255) aaTGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACgg > 2:378887/1‑133 (MQ=255) aTGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGt < 1:378887/133‑1 (MQ=255) cgcTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTAAACCGCCAACCAATCTCCCATTATTGACAAAATACGGTTAg > 2:206122/1‑133 (MQ=255) cTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATAATTAACAAGATAAGGTGAGCTGGAGCGTTCGAAGCCAGTac > 2:144322/1‑133 (MQ=255) tGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTaca < 2:446509/133‑1 (MQ=255) gAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCTCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTacac < 1:471942/133‑1 (MQ=255) tatCATTACGCCGATTGTTGAGGCTAATAAGCAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTg < 1:206122/133‑1 (MQ=255) aaGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCt > 2:255308/1‑133 (MQ=255) aTCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTc > 2:488964/1‑133 (MQ=255) aCAGGATATCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAg < 2:257149/133‑1 (MQ=255) ataaCTGAAACCTGTGCATTGCGGGGCTCAACCGCCAATCAGTCTCCCATTATTAACAAGATCCGGTTAGCTGGAGAGTTCGAACCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACtt < 2:176543/133‑1 (MQ=255) atCTGAAATCTGTCCATTCCGGTACTGAACTGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTcc < 1:159827/133‑1 (MQ=255) atCTGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTcc < 1:255308/133‑1 (MQ=255) tGAAAGCTGTCCATTGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGcc > 1:373360/1‑133 (MQ=255) tGCGGCACTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCg < 2:373360/133‑1 (MQ=255) | AAAATCAGGGTGTAGGCAAAGGGAATTGGCGTATAGGCAATGCGCTCGCATCCTGCCAGGACCGCTGAAATATCATTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCG > NC_000913/1608382‑1608638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |