Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,026	T→C	Q100Q (CAA→CAG)	yneG ←	conserved protein YneG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,026	0	T	C	100.0%	52.1 / NA	16	Q100Q (CAA→CAG)	yneG	conserved protein YneG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/10);  total (6/10)

TGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCA  >  NC_000913/1611900‑1612155                                                                                                                               |                                                                                                                                  tGTTCGCTGGCGGCGGCGAAAAATAAAGGGACCCGGGTAATGCTATTTTGTCTTTTCCCGCAATTTTCCTGGCTGATTCATCTGCACCCCCAGCCAGTGTAAGATTACCGCCACGATATAACGTTGCTGttct                                                                                                                             <  2:392364/133‑1 (MQ=255)     cGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATAGAATGTTGCTATTCTTCGc                                                                                                                         >  1:222281/1‑133 (MQ=255)      gCTGACAGCGGCGGAAAATAAAGGTCCCCTGGGAATGCTTTTTTGTCTTTTTCCGCAATTCTCATGGCTGATTCATCTGTACACCCAGCCAGTAATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCt                                                                                                                        <  2:381065/133‑1 (MQ=255)       cTGACCGCGGGGACAAATAAAGGTCCCTTGGAAATGCTTTTTTGTCGTTTTCCGCATTTTCCATGGTTGATTCATTTGTACAGCCAGCAAATGATAGTTTACCGCCACGATATAACGTTGCTGTTCTTCGCtt                                                                                                                       <  2:496194/133‑1 (MQ=255)              cggcggCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGaaa                                                                                                                <  2:7379/133‑1 (MQ=255)                  ggcAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACAcc                                                                                                            <  1:51697/133‑1 (MQ=255)                  ggaaaaaaaAGGTCCTCTGGGAATGCTTGTTTCTCGTTTTCTGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACAcc                                                                                                            <  2:222281/130‑1 (MQ=255)                             gTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGt                                                                                                 >  2:439161/1‑133 (MQ=255)                                               tttGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGAc                                                                               >  2:14643/1‑133 (MQ=255)                                                  gTCGTTTTCCGCAATACTCATGCCTGATTCGTCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAAc                                                                            <  1:231870/133‑1 (MQ=255)                                                            gCATTTCTCAGGGCGGATTCATCTCTACGCCCCGCCAGTGAAAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGAcagca                                                                  <  2:171571/133‑1 (MQ=255)                                                                               cATCTGTACAACCAGCCAGCAATAGGTTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGGGGTGGCGTGCTGa                                               <  2:82997/133‑1 (MQ=255)                                                                                  cTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCg                                            <  1:332349/133‑1 (MQ=255)                                                                                          ccagccagTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAacac                                    >  1:388368/1‑133 (MQ=255)                                                                                               cagTGATAGATTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAACACCGGAt                               >  1:477163/1‑133 (MQ=255)                                                                                                        aTTACCGCCACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTTGCGGTGGCGTGCTGAGCGATAAACACCGGATGCCCGCGCa                      >  2:36102/1‑133 (MQ=255)                                                                                                               ccACGATATAACGTTGCTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGt               >  1:254496/1‑133 (MQ=255)                                                                                                                            ttgctgttCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAACCCCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCa  <  2:388368/133‑1 (MQ=255)                                                                                                                               |                                                                                                                                  TGTTCGCTGACAGCGGCGGCAAATAAAGGTACCCTGGGAATGCTATTTTGTCGTTTTCCGCAATTCTCATGGCTGATTCATCTGTACAACCAGCCAGTGATAGATTACCGCCACGATATAACGTTGTTGTTCTTCGCTTAACGAAACACCCTGCGGAATGTTATGCCACTTAGCCAGACAACCGCGACAGCAGGTCGCGGTGGCGTGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCA  >  NC_000913/1611900‑1612155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑