Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,024	T→C	F225L (TTT→CTT)	ydfH →	DNA‑binding transcriptional repressor YdfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,024	0	T	C	100.0%	79.6 / NA	25	F225L (TTT→CTT)	ydfH	DNA‑binding transcriptional repressor YdfH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/17);  total (8/17)

CCAGCATCTTGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGG  >  NC_000913/1628897‑1629153                                                                                                                                |                                                                                                                                  ccAGCATCTTGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGc                                                                                                                              >  2:345625/1‑133 (MQ=255)  cAGCATCTTGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCg                                                                                                                             <  1:18504/133‑1 (MQ=255)       tCTTGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAagag                                                                                                                        <  1:461733/133‑1 (MQ=255)          tGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGAGACTGGCTTAGCGAAGAGTaa                                                                                                                     <  1:236046/133‑1 (MQ=255)                      tGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCctct                                                                                                         >  1:497166/1‑133 (MQ=255)                       gCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGTAAACAGCGCCTGCCTTGGCGAAGAGTAATTCATTTCCtctc                                                                                                        >  2:209490/1‑133 (MQ=255)                         ccTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTcat                                                                                                      <  1:35083/133‑1 (MQ=255)                               aaaaCGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTcatcccatc                                                                                                <  1:78635/133‑1 (MQ=255)                                     tGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGt                                                                                          <  2:356445/133‑1 (MQ=255)                                             aTGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCt                                                                                  <  2:343610/133‑1 (MQ=255)                                                          tGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCtt                                                                     >  1:266357/1‑133 (MQ=255)                                                          tGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCtt                                                                     >  2:308312/1‑133 (MQ=255)                                                          tGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTACCTCTCATACCACCCGGGGTGAGAGCCTTTGCCCCCGCCtt                                                                     >  2:222057/1‑133 (MQ=255)                                                           gCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTa                                                                    <  2:266357/133‑1 (MQ=255)                                                                       cATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTcatgcat                                                        <  1:387710/133‑1 (MQ=255)                                                                                   aTCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAg                                            >  1:404682/1‑133 (MQ=255)                                                                                      aGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGAtg                                         <  2:501936/133‑1 (MQ=255)                                                                                        cGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGAtgtg                                       >  2:420684/1‑133 (MQ=255)                                                                                           aTCCGTGCGCGAGATGCGCCAGGAGAACAGCGGCTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGc                                    <  2:202351/133‑1 (MQ=255)                                                                                            tCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCt                                   <  1:308312/133‑1 (MQ=255)                                                                                               gTGCGCCAGATCCGCCAGGAAAACAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGa                                >  1:97227/1‑133 (MQ=255)                                                                                                 gcgcCAGATCCGCCAAGAAAACAGGGACTGGCTTAGCGAAGTGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATc                              <  2:218397/133‑1 (MQ=255)                                                                                                                    aaCAGCGACTGGCTTCGCTAAGGGTAATTCCTTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCa           <  1:209490/133‑1 (MQ=255)                                                                                                                    aaCAGCGACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCa           <  1:355747/133‑1 (MQ=255)                                                                                                                          gACTGGCTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGAc     <  1:420684/133‑1 (MQ=255)                                                                                                                            cTGGCTTAGCGAAGAGTACTTCTTTTCCTCCCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAg   <  1:222057/133‑1 (MQ=255)                                                                                                                             tGGCTTAGCTAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAgg  <  1:64565/133‑1 (MQ=255)                                                                                                                                |                                                                                                                                  CCAGCATCTTGATATTTTCTCTGCCCTGCAAAAACGTGATGGCGATGCGGTAGAACGTGCAATGACGCAACATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGG  >  NC_000913/1628897‑1629153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑