| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,755,537 | T→C | intergenic (‑396/‑161) | fumD ← / → pykF | fumarase D/pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,755,537 | 0 | T | C | 92.0% | 67.3 / ‑2.0 | 25 | intergenic (‑396/‑161) | fumD/pykF | fumarase D/pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (6/17); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTC > NC_000913/1755411‑1755665 | gttttGAAACGCTGTTTTTGTTTTCCTTTTGGTTTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCt > 2:109452/2‑133 (MQ=255) aCGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGAcaca < 1:344689/133‑1 (MQ=255) cGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACaa < 1:65765/133‑1 (MQ=255) cTGTTTTTGTCTTCCCTTTGGATTCATTCCAGTGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGc < 2:130007/133‑1 (MQ=255) tttttgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGcaca < 2:121949/133‑1 (MQ=255) tttgttttCCTTTTGGATTAATTTAAGCATATTATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGcacaca < 2:161480/133‑1 (MQ=255) gttttCCTATTGGATCAATTTCCGCGTATATTGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTc < 1:131004/133‑1 (MQ=255) ttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCctc > 1:433537/1‑133 (MQ=255) ggATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCt < 2:335945/133‑1 (MQ=255) ggATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATCGTTTCAGCACTTTGGACTGTAGATCTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACGCATTCCTCTGCACGCt < 2:256959/133‑1 (MQ=255) cGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAc > 2:461360/1‑133 (MQ=255) gTATAATGCGCGCCATTTGACTCTTGAACGGTCTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc < 2:143363/133‑1 (MQ=255) gTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc < 1:282736/133‑1 (MQ=255) gTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAcc < 2:403683/133‑1 (MQ=255) tataATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCt < 2:5152/133‑1 (MQ=255) gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTagag > 1:17012/1‑133 (MQ=255) ttGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAgg < 2:433537/133‑1 (MQ=255) tGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGc < 1:376316/133‑1 (MQ=255) ttGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcac < 1:282853/129‑1 (MQ=255) ttGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTATCGATGTCACCTATCCTTAGAGCGAGGcaccacc < 1:109452/133‑1 (MQ=255) ttGAATGGCTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcac > 2:282853/1‑129 (MQ=255) tttCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGt > 2:304209/1‑133 (MQ=255) tGGACTGTAGAACTCAACGACTCAAAAACAGGCTCTTACGCTGGGCTGAGACACAAGCACACATGCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGAtt < 2:79333/133‑1 (MQ=255) tGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCGCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGAtt < 1:254834/133‑1 (MQ=255) aaCGACTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTg > 2:385142/1‑133 (MQ=255) cTCAAAAACAGGCACTCACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGccc > 2:108554/1‑133 (MQ=255) aaCAGGCACTCACGCTGGACTGAGACACAAACACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAg < 1:190904/133‑1 (MQ=255) cACGCTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTtctc < 1:108554/133‑1 (MQ=255) | TTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTC > NC_000913/1755411‑1755665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |