Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,527 A→G H47R (CAC→CGC)  yacL → UPF0231 family protein YacL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,5270AG96.7% 96.7 / NA 30H47R (CAC→CGC) yacLUPF0231 family protein YacL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/14);  minor base T (0/1);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGA  >  NC_000913/134397‑134655
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gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAGGAGGTGAAAGAAAACCTGGACTTGCTTGATGAAGTGTAACAAACAGcgcgcg                                                                                                                                >  2:240486/1‑133 (MQ=255)
              tatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGc                                                                                                                  >  1:327506/1‑133 (MQ=255)
                ttACCGGACTGGTAACGGAGCGAATGTCCAAGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAGGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGa                                                                                                                <  1:240486/133‑1 (MQ=255)
                        gTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCt                                                                                                        >  1:18287/1‑133 (MQ=255)
                        gTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCt                                                                                                        >  1:77425/1‑133 (MQ=255)
                        gTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCt                                                                                                        >  1:318169/1‑133 (MQ=255)
                        gTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGAGGAACAAGGAGCGCGCGCACTGAAAAGTAGCGAACGGTCCt                                                                                                        >  2:165272/1‑133 (MQ=255)
                           gTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGc                                                                                                     <  1:58065/133‑1 (MQ=255)
                                 gTGCGTATCTCCATGTGGTATGAAGTGGTCTGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACggg                                                                                               <  1:165272/133‑1 (MQ=255)
                                  tGCGTATGTCGATGGAGCATGAAGCGGTAGGGTACTGGTTTCATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                                                                              <  2:179913/133‑1 (MQ=255)
                                     gTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagg                                                                                           >  2:399440/1‑133 (MQ=255)
                                      tATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                                                                          >  2:387444/1‑133 (MQ=255)
                                      tATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                                                                          <  1:124800/133‑1 (MQ=255)
                                      tATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                                                                          <  1:399440/133‑1 (MQ=255)
                                        tGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggca                                                                                        <  2:318169/133‑1 (MQ=255)
                                          tCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGTAACGGGTAGGGCATg                                                                                      >  1:179913/1‑133 (MQ=255)
                                                    aTGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCt                                                                            <  2:3491/133‑1 (MQ=255)
                                                           ggTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCAAGACTACACCCTGtggatg                                                                     >  1:212081/1‑133 (MQ=255)
                                                            gccGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGGAGCGAACGGTCCTGCGAACGGGCAGTGCATGAAGACACCCTGtggatgg                                                                    >  2:333781/3‑133 (MQ=255)
                                                            gTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                                                                    >  2:351152/1‑133 (MQ=255)
                                                             tCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgga                                                                   <  2:77425/133‑1 (MQ=255)
                                                             tCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgga                                                                   <  2:18287/133‑1 (MQ=255)
                                                             tCGGGCACTAGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGTAACAAGCAGCGCGCGCACTTAAAGGTAGCTAACGGTCCCGGCAACGGGCAGGGCATGAAAACACCCTGtggatgga                                                                   <  2:76939/133‑1 (MQ=255)
                                                                      ggTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga                                                          >  2:82400/1‑133 (MQ=255)
                                                                                 gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCGTGAATACAGCCTGTGGGTGGACGGTAAAGACGTGATGGTTc                                               >  2:151693/1‑133 (MQ=255)
                                                                                 gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTc                                               >  2:109032/1‑133 (MQ=255)
                                                                                 gagGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTc                                               >  1:379252/1‑133 (MQ=255)
                                                                                   ggTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCGCGCACTCAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTcgc                                             >  1:507160/1‑133 (MQ=255)
                                                                                         agaaaACCTGGCCTTGCTTGATGAAGTGTAACAAGCAGCGCTCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAAt                                       <  2:174555/133‑1 (MQ=255)
                                                                                                                   tGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAAt             <  1:387444/133‑1 (MQ=255)
                                                                                                                   tGGAACAAGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAAt             <  1:333781/133‑1 (MQ=255)
                                                                                                                         aaGCAGCGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAGGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAaga       <  2:379252/133‑1 (MQ=255)
                                                                                                                           gcagcGCGCGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAgg     <  2:327506/133‑1 (MQ=255)
                                                                                                                              gcgcgcgcACTGAAAGGTAGCGAACGGTACTGCCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGa  <  2:212081/133‑1 (MQ=255)
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GAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGATGGTTCGCGCCAATCAACTGGAATTCGCTGGCGATGAAATGGAAGAGGGGA  >  NC_000913/134397‑134655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑