Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,564	G→A	V48V (GTG→GTA)	lpp →	murein lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,564	0	G	A	96.2%	78.9 / ‑4.3	27	V48V (GTG→GTA)	lpp	murein lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/16);  total (9/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTG  >  NC_000913/1757434‑1757690                                                                                                                                   |                                                                                                                               aaCTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTaaa                                                                                                                              >  2:24668/1‑133 (MQ=255)      gTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCaa                                                                                                                         >  1:2332/1‑133 (MQ=255)      gTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCaa                                                                                                                         >  2:312057/1‑133 (MQ=255)            ggCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGtt                                                                                                                   >  1:323723/1‑133 (MQ=255)                             ttCTACTCTGCTGACAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACCTAAACGCAATGCGTTCCGACGCTCAGgctgct                                                                                                  >  2:191990/1‑133 (MQ=255)                                tACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTaaa                                                                                               >  1:88788/1‑133 (MQ=255)                                           cAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGc                                                                                    <  1:102769/133‑1 (MQ=255)                                           cAGGTTGCCCCAGCACCGCTAATATCGATCCGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGc                                                                                    <  1:139956/133‑1 (MQ=255)                                                           cccTAAAATTCATCAGCTGTCTTCCGTCGTTCAGACTCTGACCGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGt                                                                    <  2:241290/131‑1 (MQ=255)                                                           cGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGt                                                                    <  1:338871/133‑1 (MQ=255)                                                            gCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTc                                                                   >  2:63623/1‑133 (MQ=255)                                                            gCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTc                                                                   <  1:387777/133‑1 (MQ=255)                                                               aaaaTCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTgg                                                                >  2:425778/1‑133 (MQ=255)                                                                      aTCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACAt                                                         <  2:430000/133‑1 (MQ=255)                                                                      aTCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACAt                                                         <  1:425778/133‑1 (MQ=255)                                                                        cAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATgg                                                       <  1:47654/133‑1 (MQ=255)                                                                                ttctGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTaaa                                               <  1:273660/133‑1 (MQ=255)                                                                                   tGACGTTCTGACTCTGAATGCTAAAGTTGATCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAc                                            <  1:248718/133‑1 (MQ=255)                                                                                    gACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATAcc                                           <  1:38280/133‑1 (MQ=255)                                                                                        ttCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCaa                                       >  2:421949/1‑133 (MQ=255)                                                                                        ttCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCaa                                       <  1:24668/133‑1 (MQ=255)                                                                                          cAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGt                                     <  2:88788/133‑1 (MQ=255)                                                                                             aCTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAAt                                  <  1:312057/133‑1 (MQ=255)                                                                                             aCTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAAt                                  <  2:392928/133‑1 (MQ=255)                                                                                               tctGAACGCTAAAGTTGACCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAg                                <  2:41926/133‑1 (MQ=255)                                                                                                                aCCAGCTGAGCAACGGCGTAAACGCAATGCGTTCCGACGTCCAGGCTGCTTAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGaaaa               <  2:316556/133‑1 (MQ=255)                                                                                                                aCCAGCTGAGCAACGACGTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGaaaa               <  2:20417/133‑1 (MQ=255)                                                                                                                 ccAGCTGACCAACGACGTTAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGaaaaa              <  1:191990/133‑1 (MQ=255)                                                                                                                             cgacgTAAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAATACCTGTGAAGGGAAAAATGGCGCACATtg  >  1:252594/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                               AACTGGTACTGGGCGCGGTAATCCTGGGTTCTACTCTGCTGGCAGGTTGCTCCAGCAACGCTAAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGACCAGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTG  >  NC_000913/1757434‑1757690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑