Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,783,511	G→A	A179T (GCT→ACT)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,783,511	0	G	A	100.0%	66.0 / NA	22	A179T (GCT→ACT)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

TCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGT  >  NC_000913/1783383‑1783633                                                                                                                                 |                                                                                                                           tCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTCTCGTCGAGGACAATCTGACTcc                                                                                                                        >  1:148546/1‑133 (MQ=255) tCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTCTCGGCGGGGACAAACTGACTcc                                                                                                                        >  1:237674/1‑133 (MQ=255)       aGTGTCAGGCGAAAACGTTCTTTGTACCTACGTTGTTTAAATAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCAc                                                                                                                  <  2:148546/133‑1 (MQ=255)       aGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCAc                                                                                                                  <  1:264452/133‑1 (MQ=255)          gtCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACctc                                                                                                               >  2:422726/1‑133 (MQ=255)           tCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAAGCCTGCCGCTGCAAAATCAGCTCCCGCAACTACAACAAATTGACGGCGTGGACAAACTGACTCCCGCCACctct                                                                                                              <  1:22505/133‑1 (MQ=255)             aGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACGAACAGACTCCCGCTACCTCTTc                                                                                                            >  1:211240/1‑133 (MQ=255)             aGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTc                                                                                                            >  1:288787/1‑133 (MQ=255)              ggCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTcc                                                                                                           <  2:446109/133‑1 (MQ=255)                     aTGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCAt                                                                                                    <  2:288787/133‑1 (MQ=255)                                    aCGTTGTTTATACAATCGCGTCCGATAGTTTTCATCCTGCCACTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCg                                                                                     <  2:156982/133‑1 (MQ=255)                                    aCGTTGTTTAAACAAGCGCGTCTGGTAGATATAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCg                                                                                     <  1:230543/133‑1 (MQ=255)                                      gttgttTAAAAAAACCCGTCCGGTAGATCTAACCCTGCCGCCGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGcc                                                                                   <  2:211240/133‑1 (MQ=255)                                                   acGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCAc                                                                      >  2:475862/1‑133 (MQ=255)                                                                  ttAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATaa                                                       <  1:387854/133‑1 (MQ=255)                                                                                   aaaTCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGaa                                      >  2:58908/1‑133 (MQ=255)                                                                                                       aacaaATTGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTAc                  >  2:264989/1‑133 (MQ=255)                                                                                                             ttGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGGTGAATTAACTGCGGCGCTGTTGACCTCCgg            >  1:245541/1‑133 (MQ=255)                                                                                                              tGTCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGa           >  2:297145/1‑133 (MQ=255)                                                                                                                tCGGCGTGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAAc         >  2:395738/1‑133 (MQ=255)                                                                                                                      tGGACAAACTGACTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAggg   >  1:125232/1‑133 (MQ=255)                                                                                                                       ggACAAACTCACTCCCGCCACCTCTTCCGTCTTATTAAGTCTGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGt  <  2:245541/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                           TCAATAAGTGTCAGGCAAAAATGTTCTTTGCACCGACGTTGTTTAAACAAACGCGTCCGGTAGATTTAATCCTGCCGCTGCAAAATCAGCTTCCACAACTACAACAAATTGTCGGCGTGGACAAACTGGCTCCCGCCACCTCTTCCCTCTCATTAAGTCAGATTATCGCCGACAATACCTCACTGACCACGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGT  >  NC_000913/1783383‑1783633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑