Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,814,387	G→A	G174D (GGC→GAC)	katE →	catalase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,814,387	0	G	A	100.0%	85.4 / NA	27	G174D (GGC→GAC)	katE	catalase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

GATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGGCTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATGCGCATAAATTCCCCGAT  >  NC_000913/1814258‑1814514                                                                                                                                  |                                                                                                                                gATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGActc                                                                                                                              <  1:14579/133‑1 (MQ=255)  aTCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGGTTCTCTAGCGTGCAGGGTGGTTCTGActct                                                                                                                             >  1:144304/1‑133 (MQ=255)         gCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCCACCGTTCAGGGTGGTGCTGACTCTGCTGATa                                                                                                                      <  1:28671/133‑1 (MQ=255)                        cAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATcc                                                                                                       <  1:9046/133‑1 (MQ=255)                          gCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGGGCTGACTCTGCTGATACCGCGCGTGATATCCGt                                                                                                     >  2:403165/1‑133 (MQ=255)                               ataAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCTCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCtt                                                                                                >  2:125890/1‑133 (MQ=255)                                      cTTAAGCGATATTACCAAAGCGGATTTCCTCTCTGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGccacc                                                                                         <  2:359593/133‑1 (MQ=255)                                      cTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGccacc                                                                                         <  1:125890/133‑1 (MQ=255)                                      cTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGccacc                                                                                         <  2:25853/133‑1 (MQ=255)                                          aGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATAGCGTCCGTTATATCCGTAGCTTTGCCACCAAGt                                                                                     >  2:219468/1‑133 (MQ=255)                                           gCGATATTACCAAAGTGGCTTTTCTCTCAGCTCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGtt                                                                                    <  1:159734/133‑1 (MQ=255)                                           gCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGtt                                                                                    <  1:125391/133‑1 (MQ=255)                                                         gCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAgg                                                                      >  2:44743/1‑133 (MQ=255)                                                         gCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAgg                                                                      >  2:344193/1‑133 (MQ=255)                                                         gCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAgg                                                                      <  2:285624/133‑1 (MQ=255)                                                                  ctctCAGATCTGAACCAAATCACCTCAGCATTCGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTg                                                             <  2:144304/133‑1 (MQ=255)                                                                         aTCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGt                                                      >  1:305625/1‑133 (MQ=255)                                                                                   aaTCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAac                                            >  2:323693/1‑133 (MQ=255)                                                                                         cccAGTATATGTACGATTATCTTCCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCa                                      <  1:219468/133‑1 (MQ=255)                                                                                            aGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAAtc                                   >  1:2370/1‑133 (MQ=255)                                                                                               aTTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAAtcttc                                <  1:323693/133‑1 (MQ=255)                                                                                                        tttCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTAGGCAATAGCACGCCTATCTTCTTTATCCAg                       >  1:152862/1‑133 (MQ=255)                                                                                                         ttCTCTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAgg                      >  1:323725/1‑133 (MQ=255)                                                                                                          tctcTACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGa                     <  1:344193/133‑1 (MQ=255)                                                                                                            tctACCGTTCAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATg                   <  1:403165/133‑1 (MQ=255)                                                                                                                     cAGGGTGGTGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATGCGCATAAAt          >  2:455822/1‑133 (MQ=255)                                                                                                                        ggtggtGCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATGCGCATAAATTcc       <  2:305625/133‑1 (MQ=255)                                                                                                                             tgCTGACTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATGCGCATAAATTCCCCGAt  >  2:36841/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                GATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTCAGGGTGGTGCTGGCTCTGCTGATACCGTGCGTGATATCCGTGGCTTTGCCACCAAGTTCTATACCGAAGAGGGTATTTTTGACCTCGTTGGCAATAACACGCCAATCTTCTTTATCCAGGATGCGCATAAATTCCCCGAT  >  NC_000913/1814258‑1814514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑