| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,836,865 | C→A | L265M (CTG→ATG) | ynjB → | putative ABC transporter periplasmic binding protein YnjB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,836,865 | 0 | C | A | 86.7% | 39.9 / NA | 15 | L265M (CTG→ATG) | ynjB | putative ABC transporter periplasmic binding protein YnjB |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (8/5); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCATCCGTATTTGTGGCGCGAAGGAAAGGATTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGCTGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCATTTCGTCACCATTCCTGCCAACGCG > NC_000913/1836772‑1836993 | ctgcATCCGTATTTGTGGCGCGAAGGAAAGGATTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCa > 2:41914/1‑133 (MQ=255) gcATCCGTATTTGTGGCGCGAAGGAAAGGATTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAgc > 1:493199/1‑133 (MQ=255) gcgAAGGAAAGGATTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAg < 2:456284/133‑1 (MQ=255) gcgAAGGAAAGGATTTCCCGCCTTAACCCGCGCGGATGGATCCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAg < 2:244120/133‑1 (MQ=255) aaggaaaggaTTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAg > 1:288767/1‑133 (MQ=255) ggaaaggaTTTCCCGCCTTCACCCGCGGGGATGGATGCTCTGCTGAAAGCCGGCCCATTGCGCCTGTCGCTGAACTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGtt < 2:463332/133‑1 (MQ=255) ggaaaggaTTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGtt < 1:4789/133‑1 (MQ=255) ttCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTcgc < 2:288767/133‑1 (MQ=255) gcgGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATCTGCCTGCAGGCACTTAGAGTTTTGGGTTTCGCGAGGGgatg > 2:144198/1‑133 (MQ=255) tgctgAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCa > 1:46440/1‑133 (MQ=255) gctgAAAGCCTGCACCTTGCGCCTATCGCTGATCTTTAACCTCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCAt < 2:137004/133‑1 (MQ=255) ctgAAAGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCAtt > 2:326668/1‑133 (MQ=255) aGCCGGCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCATTTCGTc > 2:485603/1‑133 (MQ=255) gCACATTGCGCCTGTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGGGCATTTCGTCACCAt > 2:187623/1‑133 (MQ=255) gTCGATGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCATTTCGTCACCATTCCTGCCAAcgcg < 1:41914/133‑1 (MQ=255) | CTGCATCCGTATTTGTGGCGCGAAGGAAAGGATTTCCCGCCTTCACCCGCGCGGATGGATGCTCTGCTGAAAGCCGGCACATTGCGCCTGTCGCTGACCTTTAACCCCGCGCATGCGCAGCAAAAAATCGCCAGCGGCGATTTGCCTGCAAGCAGTTACAGTTTTGGCTTTCGCGAGGGGATGATTGGCAACGTGCATTTCGTCACCATTCCTGCCAACGCG > NC_000913/1836772‑1836993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |