| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,855,920 | A→G | L18L (TTG→CTG) | ydjG ← | NADH‑dependent methylglyoxal reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,855,920 | 0 | A | G | 96.3% | 82.1 / NA | 27 | L18L (TTG→CTG) | ydjG | NADH‑dependent methylglyoxal reductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (13/13); minor base C (1/0); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCG > NC_000913/1855799‑1856047 | ccTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTcgcg < 1:8820/133‑1 (MQ=255) cTGGCGCAGTGTCAACCAGATTAACGCCCCAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGa < 2:146575/133‑1 (MQ=255) gcgcAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCCATGGCCCATGTCCCCACCCCCCGTCGCGAAAg > 2:239902/1‑133 (MQ=255) gcgcAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAg > 1:127698/1‑133 (MQ=255) gcgcAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAAGGGCCCATTTCCACATCCCCATCCGCGAAAg > 1:155107/1‑133 (MQ=255) gcgcAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAAGGGCCCATATCCGCAGCCCCATGCGCGAAAg > 1:133476/1‑133 (MQ=255) gTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTaa > 2:352504/1‑133 (MQ=255) tcaGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGt < 2:419141/133‑1 (MQ=255) aTTAATGCCACAACGATGGGCTTCGAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTtgtg > 1:345243/1‑133 (MQ=255) aTTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGGCCCATTCTCGACAGAGTAATAGCCGTtgtg > 1:159227/1‑133 (MQ=255) tAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGcc < 1:32306/133‑1 (MQ=255) aTGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCttt > 1:288495/1‑133 (MQ=255) ttCAAGAATCTTATCCATACATATTTGCTGATCGAGATCGCTATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCAtt < 2:133476/133‑1 (MQ=255) aaGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGc > 1:497580/1‑133 (MQ=255) aaGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGc < 2:288495/133‑1 (MQ=255) gaaTCAATACATATTCGCCGATCGCGATTGCCCTTCCATGCCGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCAtt < 2:234399/131‑1 (MQ=255) tATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTa > 2:484013/1‑133 (MQ=255) aTACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCtt < 2:127698/133‑1 (MQ=255) atTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAtt > 2:275924/1‑133 (MQ=255) gCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAttcttc > 2:270189/1‑133 (MQ=255) cAGGACCGCCGCCAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCt < 1:484013/133‑1 (MQ=255) ccgccAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTa > 1:222922/1‑133 (MQ=255) ccgccAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGAATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCGGCAATTGTTCGGCCGGCTTTCGGTTTTTTa > 1:144939/1‑133 (MQ=255) gccAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg < 2:222922/133‑1 (MQ=255) gccAATGGCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCATTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg < 2:497580/133‑1 (MQ=255) gCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTAca < 1:496599/126‑2 (MQ=255) gCCCATGTCCCCAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTAca > 2:496599/1‑125 (MQ=255) ccccAGCCGCATTCGTGACAGCATAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg < 1:239902/133‑1 (MQ=255) ccccAGCCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg < 1:10190/133‑1 (MQ=255) | CCTGGCGCAGTATCAATCAGATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCG > NC_000913/1855799‑1856047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |